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You are here: EPA Home Research NCEA HERO Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Muta(TM)Mouse lung epithelial cells

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196321 
Journal Article 
Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Muta(TM)Mouse lung epithelial cells 
Jacobsen, NR; Pojana, G; White, P; Møller, P; Cohn, CA; Korsholm, KS; Vogel, U; Marcomini, A; Loft, S; Wallin, H 
2008 
Environmental and Molecular Mutagenesis
ISSN: 0893-6692
EISSN: 1098-2280 
49 
476-487 
English 
WOS:000257980200008 
Viability, cell cycle effects, genotoxicity, reactive oxygen species production, and mutagenicity of C(60) fullerenes (C(60)) and single-walled carbon nanotubes (SWCNT) were assessed in the FE1-Mutatrade markMouse lung epithelial cell line. None of these particles induced cell death within 24 hr at doses between 0 and 200 microg/ml or during long-term subculture exposure (576 hr) at 100 microg/ml, as determined by two different assays. However, cell proliferation was slower with SWCNT exposure and a larger fraction of the cells were in the G1 phase. Exposure to carbon black resulted in the greatest reactive oxygen species generation followed by SWCNT and C(60) in both cellular and cell-free particle suspensions. C(60) and SWCNT did not increase the level of strand breaks, but significantly increased the level of FPG sensitive sites/oxidized purines (22 and 56%, respectively) determined by the comet assay. The mutant frequency in the cII gene was unaffected by 576 hr of exposure to either 100 microg/ml C(60) or SWCNT when compared with control incubations, whereas we have previously reported that carbon black and diesel exhaust particles induce mutations using an identical exposure scenario. These results indicate that SWCNT and C(60) are less genotoxic in vitro than carbon black and diesel exhaust particles. 
Comet assay; FE1-MML; in vitro; mutation; oxidative damage 
Animals 
Cell Cycle 
Cell Line 
Cell Proliferation 
Cell Survival 
Comet Assay 
DNA Damage 
DNA Mutational Analysis 
Epithelial Cells 
Fullerenes 
Lung 
Mice 
Mutagenicity Tests 
Nanotubes, Carbon 
Reactive Oxygen Species 
 

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