Mutagenicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluoro-n-decanoic acid in L5178Y mouse-lymphoma cells
Authors: Rogers, AM; Andersen, ME; Back, KC
Mutation Research 105:445-449.
HERO ID: 3858719
The toxicity and mutagenicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (1746016) (TCDD) and perfluoro-n-decanoic-acid . . .
The toxicity and mutagenicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (1746016) (TCDD) and perfluoro-n-decanoic-acid (335762) (PFDA) were examined using the mouse lymphoma cell line L5178Y. The cells were treated for 24 or 48 hours with 0.001 to 500 micrograms per milliliter (microg/ml) PFDA or TCDD respectively. Effects on cells in suspension and soft agar were considered. PFDA treatment had no effect on cell growth in suspension at concentrations below 50microg/ml and caused cell lysis at doses greater than 100microg/ml. TCDD had no effect on suspension growth at any concentration, but doses greater than 0.01microg/ml resulted in the formation of larger, less discrete cloning and reduced plating efficiency in soft agar experiments. PFDA at a dose of 0.5microg/ml also reduced plating efficiency. Five selective systems were used for testing mutation induction. These included ouabain, excess thymidine, methotrexate, cytosine-arabinoside, and thioguanine. TCDD significantly increased the frequency of mutations in the methotrexate, excess thymidine, and thioguanine systems but had no significant effect on the other two selective systems. PFDA treatment resulted in no significant mutation induction in any of the selective systems used. The authors concluded that TCDD but not PFDA was a direct acting mutagen in L5178Y cells.