Prevention of neutrophil-mediated injury to endothelial cells by perfluorochemical
Authors: Babbit, DG; Forman, MB; Jones, R; Bajaj, AK; Hoover, RL
The American Journal of Pathology 136:451-460.
HERO ID: 3859496
BIOSIS COPYRIGHT: BIOL ABS. Myocardial salvage after reperfusion may be limited by neutrophil-mediated . . .
BIOSIS COPYRIGHT: BIOL ABS. Myocardial salvage after reperfusion may be limited by neutrophil-mediated microvascular damage. The effect of the perfluorochemical, Fluosol-DA, and its various components on neutrophil adherence, cytotoxicity, and proteolytic enzyme release was examined on sheep large and small vessel endothelial cells in vitro. Cells were studied under normoxic (N) and anoxic conditions (A). Various concentrations of Fluosol (10%, 25%, and 50%) significantly reduced neutrophil adherence under both experimental conditions (mean 22 | 3.25% versus 7 | 0.8% (N) and 20 | 3.2% versus 7.5 | 0.9% (A); P and lt; 0.01). The perfluorocarbons, perfluorodecalin (PFD), and perfluoro-tripropylamine (PFTP) in a 50 volume/percent concentration exhibited profound effects on adherence, particularly on cells subjected to anoxia (51% and 69% reduction in adherence, respectively; P and lt; 0.01). No effect on adherence was observed with other components including the detergent, pluroinc F68. A 25% reduction (P and