Health & Environmental Research Online (HERO)


PFBA (375-22-4)


798 References Were Found:

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Trace Analysis Of Amines And Isocyanates Using Glass Capillary Gas Chromatography And Selective Detection. II. Determination Of Aromatic Amines As Perfluorofatty Acid Amides

Authors: Skarping, G; Renman, L; Dalene, M (1983) HERO ID: 2229363

[Less] Gas chromatography (GC) for the trace analysis of aromatic amines was developed. The chromatograph . . . [More] Gas chromatography (GC) for the trace analysis of aromatic amines was developed. The chromatograph was equipped with an on column injection system and a thermionic specific detector. Aromatic amines were derivatized to their corresponding amides by reaction with a perfluorofatty acid anhydride. The amides were separated on a capillary column and detected by a thermionic specific detector. Amines investigated included aniline (62533), 2,6-toluenediamine (823405), 2,4-toluenediamine (95807), 2,4-diaminoanisole (615054), alpha-naphthylamine (134327), beta-naphthylamine (91598), 4-aminobiphenyl (92671), 1,5-naphthalenediamine (2243621), benzidine (92875), methylenedianiline (101779), o-tolidine (95534), methylene-bis-o-chloroaniline (27342752), and o-dianisidine (119904). Standard solutions of amines were prepared and amides were obtained by adding heptafluorobutyric-acid anhydride (HA), pentafluoropropionic-acid anhydride (PFA), monochloroacetic-acid anhydride (MCA), or acetic-acid anhydride (AA). The thermionic detector was optimized for maximum sensitivity by adjusting the hydrogen, air, and make up gas flow rates during injection of the derivatives. Quantitative analysis was based on peak area measurement. AA and MCA gave amides with much higher elution temperatures than the other anhydrides; AA was not suitable as an acylating agent because of the high elution temperatures and a tendency to cause tailing peaks at picogram concentrations. PFA and HA were suitable for general application, and MCA was useful for trace analysis of amines that did not have high boiling points. Injection of aromatic amine derivatives from PFA in toluene had a reproducibility of 0.8 to 1.7 percent. Columns that were not polar gave lower elution temperatures and greater resolution. Helium was the better carrier gas. The authors conclude that trace analysis of aromatic amines using capillary GC and a thermionic specific detector is a useful method for assaying amines.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

GLC of morphine and co-extractants in the rapid analysis of post mortem blood

Author: Lewis, KW (1983) Journal of Chromatographic Science 21:521-523. HERO ID: 3858414

[Less] A qualitative study of over 70 drugs and related compounds was carried out. The study incorporated a . . . [More] A qualitative study of over 70 drugs and related compounds was carried out. The study incorporated a recognised procedure for the analysis of morphine in post mortem blood. Stock solutions of the compounds were reacted with heptafluorobutyric acid anhydride and the derivatives were examined by gas-liquid chromatography on an OV-17 column using electron capture detection. The results were tabulated with respect to retention time, minimum detectable amount, and reactivity.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

alpha-N-acetyl-beta-endorphin1-26 from the neurointermediary lobe of the rat pituitary: isolation, purification, and characterization by high-performance liquid chromatography

Authors: Bennett, HP; Browne, CA; Solomon, S (1983) Analytical Biochemistry 128:121-129. HERO ID: 3858400

[Less] The neurointermediary lobes from 190 rat pituitaries were homogenized in an acidic medium which inhibits . . . [More] The neurointermediary lobes from 190 rat pituitaries were homogenized in an acidic medium which inhibits peptidase activity and maximizes the solubilization of undamaged peptides. Octadecylsilyl-silica (ODS-silica) was used to extract the supernatant of the tissue homogenate. The ODS-silica eluate, now largely protein and salt free, was subjected to reversed-phase high-performance liquid chromatography (HPLC) employing 0.1% trifluoroacetic as counter ion. The column eluates were monitored for beta-endorphin immunoreactivity. Five immunoreactive components were observed. The most hydrophobic of these was repurified on the same HPLC column using 0.13% heptafluorobutyric acid as counter ion. Characterization of the purified peptide by gel permeation HPLC, amino acid analysis, and tryptic fragmentation indicated that it corresponded in structure to alpha-N-acetyl-beta-endorphin1-26. Amino acid analysis of the native peptide and its trypsin and carboxypeptidase fragments indicated that an alanyl residue occupies position 26. This finding is in contrast to the sequence predicted for the beta-lipotropin/corticotropin precursor by recombinant DNA techniques which suggests that the 26th residue of the beta-endorphin molecule should be valine.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Identification of testosterone and progesterone in hemolymph of larvae of the fleshfly Sarcophaga bullata

Authors: De Clerck, D; Eechaute, W; Leusen, I; Diederik, H; De Loof, A (1983) General and Comparative Endrocrinology 52:368-378. HERO ID: 3858413

[Less] Testosterone- and progesterone-like substances were detected by radioimmunoassay (RIA) in chromatographed . . . [More] Testosterone- and progesterone-like substances were detected by radioimmunoassay (RIA) in chromatographed extracts of hemolymph from larvae of Sarcophaga bullata (S.B.). Gas chromatographic (GC) analysis after heptafluorobutyric acid (HFBA) derivation of hemolymph extracts, purified by paper and silica gel column chromatography, showed a peak in the GC recordings with the same retention time as the HFBA derivative of pure testosterone. A testosterone concentration of 92 ng/100 ml hemolymph was found by GC; the concentration of progesterone, calculated on the basis of the RIA, was about two times higher. After preparing the o-pentafluorobenzyloxime (OPFB)-heptafluorobutyryl ester (HFB) derivatives of the hemolymph extracts, negative ion chemical ionization capillary gas chromatography-mass spectrometry (NCI/GC-MS) proved that hemolymph of larvae of the fleshfly Sarcophaga bullata indeed contains testosterone and progesterone. Several metabolites and precursors of the latter compounds could also be detected during the NCI/GC-MS analyses. Estrogens could not be traced by any of the methods we used. This is the first time that these steroids have been identified in insect hemolymph. These results add interesting perspectives for comparative endocrinology.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Isolation of pituitary peptides by reversed-phase high-performance liquid chromatography. Expansion of the resolving power of reversed-phase columns by manipulating pH and the nature of the ion-pairing reagent

Author: Bennett, HP (1983) Journal of Chromatography 266:501-510. HERO ID: 3858415

[Less] The reversed-phase liquid chromatographic behaviour of pituitary peptides at various pH values was compared. . . . [More] The reversed-phase liquid chromatographic behaviour of pituitary peptides at various pH values was compared. The retention times of peptides at pH 1, 3 and 4.5 were essentially as expected for acidic solvent systems. However, at pH 5.5 or 7, radically different elution characteristics were observed. This is probably brought about by the increase in polarity that accompanies ionization of the peptide carboxyl groups at higher pH values. Basic hydrophobic ion-pairing reagents, such as tetrabutylammonium ion, can be used to maximize differences in polarity. We have previously shown that the use of acidic hydrophobic ion-pairing reagents at low pH (e.g. trifluoroacetic and heptafluorobutyric acids) enhances the basic polarity of peptides. The combination of both approaches facilitated the reversed-phase purification of posterior pituitary glycopeptide and alpha-N-acetyl-beta-endorphin1-27 from an extract of 200 rat neurointermediate pituitaries. The separation of these two peptides at low pH, which is particularly difficult, was achieved by the sequential use of solvent systems containing trifluoroacetic acid (0.1%), heptafluorobutyric acid (0.13%) and finally triethylamine acetate (0.01 M, pH 5.5).

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Procedure for the gas chromatographic-mass spectrometric confirmation of some exogenous growth-promoting compounds in the urine of cattle

Authors: Tuinstra, LG; Traag, WA; Keukens, HJ; van Mazijk, RJ (1983) Journal of Chromatography 279:533-542. HERO ID: 2325573

[Less] As gas chromatography-mass spectrometry is the most conclusive confirmation technique available today . . . [More] As gas chromatography-mass spectrometry is the most conclusive confirmation technique available today for the detection of ppb levels of anabolics in the urine of cattle, the following procedure was used. The urine is hydrolysed with Helix pomatia intestinal juice, extracted, and the extract cleaned by gel-permeation chromatography or with Extrelut. In one fraction are eluted diethylstilboestrol, dienoestrol, hexoestrol, methyltestosterone, ethinyloestradiol, zeranol and trenbolone. This fraction is injected into a two-dimensional high-performance liquid chromatography system. From the first column, the fraction containing the above-mentioned compounds is transferred to the second column, where a separation into two fractions is obtained. The first fraction contains zeranol and trenbolone, the second fraction the stilbenes, methyltestosterone and ethinyloestradiol. In general, the compounds are derivatized with an N,O-bis (trimethylsilyl)trifluoroacetamide-trimethylchlorosilane mixture to a trimethylsilyl derivative. With stilbene, confirmatory derivatization into heptafluorobutyryl derivatives is necessary. In combination with a Finnigan 4000/INCOS system, a CP-Sil-5 CB and a CP-Sil-19 CB capillary are used for final confirmation. Two capillaries with different polarities are necessary to overcome problems with possible interferences from compounds extracted from the urine. Recoveries at the ppb level are better than 80%.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Gas--liquid chromatographic resolution and assay of tocainide enantiomers using a chiral capillary column and study of their selective disposition in man

Authors: Mcerlane, KM; Pillai, GK (1983) Journal of Chromatography 274:129-138. HERO ID: 2325574

[Less] Tocainide is a new antiarrhythmic agent that is used clinically as the racemic mixture. In order to . . . [More] Tocainide is a new antiarrhythmic agent that is used clinically as the racemic mixture. In order to study the disposition of the individual enantiomers in man, a gas--liquid chromatographic assay was developed based on the resolution of the R-(-)- and S-(+)-enantiomers as their heptafluorobutyryl derivatives on a capillary column coated with a chiral stationary phase. Two healthy male volunteers ingested an oral solution dose of racemic tocainide hydrochloride at a dose of 3 mg/kg and plasma and urine were collected at intervals for up to 54 h. Analysis of the plasma samples revealed a stereoselective disappearance of the R-(-)-enantiomer, such that the apparent half-life for the S-(+)- and R-(-)-enantiomers were 25.6 and 20.5 h, respectively in one subject and 11.1 and 9.0 h in the second subject. Similar relationships were observed in urine, where the ratio of the S-(+)- to R-(-)-enantiomers varied from 0.98 in 1 h to 3.03 in the 54-h samples in one subject over the same time period.

Book/Book Chapter
Book/ Chapter

Risk assessment in the federal government: Managing the process

Author: NRC (1983) Washington, DC: National Academy Press. HERO ID: 194806

[Less] The regulation of potentially hazardous substances has become a controversial issue. This volume evaluates . . . [More] The regulation of potentially hazardous substances has become a controversial issue. This volume evaluates past efforts to develop and use risk assessment guidelines, reviews the experience of regulatory agencies with different administrative arrangements for risk assessment, and evaluates various proposals to modify procedures. The book's conclusions and recommendations can be applied across the entire field of environmental health.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Profiling of urinary medium-sized peptides in normal and uremic urine by high-performance liquid chromatography

Authors: Mabuchi, H; Nakahashi, H (1982) Journal of Chromatography 233:107-113. HERO ID: 3858401

[Less] This report describes the profiling of medium-sized peptides in both normal and uremic urine by ion-pair . . . [More] This report describes the profiling of medium-sized peptides in both normal and uremic urine by ion-pair reversed-phase high-performance liquid chromatography using an acetonitrile--heptafluorobutyric acid solvent system as eluent. Several medium-sized peptide peaks could be detected in both normal and uremic urine at low picomole level by using post-column fluorescence derivatization with fluorescamine. Contrary to expectation, uremic urine contained slightly larger amounts of medium-sized peptides compared with normal urine.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Two forms of murine epidermal growth factor: rapid separation by using reverse-phase HPLC

Authors: Burgess, AW; Knesel, J; Sparrow, LG; Nicola, NA; Nice, EC (1982) Proceedings of the National Academy of Sciences 79:5753-5757. HERO ID: 3858402

[Less] Epidermal growth factor (EGF) has been isolated from acid extracts of C57BL6/J mouse submaxillary glands . . . [More] Epidermal growth factor (EGF) has been isolated from acid extracts of C57BL6/J mouse submaxillary glands by using hydrophobic chromatography. High yields of EGF in large amounts (10 mg) can be isolated reliably from the acid extract of the glands in less than 4 hr. The reverse-phase HPLC techniques used to purify the EGF initially yielded what appeared to be a single homogeneous EGF molecule. However, ion pairing reagents (e.g., heptafluorobutyric acid) altered the chromatographic properties, revealing two distinct species: EGF-alpha and EGF-beta. The apparent molecular weights, isoelectric points, and antigenic properties of EGF-alpha and EGF-beta were identical, and both forms stimulated a mitogenic response in 3T3 cells. Analysis of different preparations of purified EGF (commercial and experimental) indicated the presence of EGF-alpha and EGF-beta in constant proportion. Previous EGF binding studies must have used mixtures of 125I-labeled EGF-alpha and 125I-labeled EGF-beta. The two molecules appear to compete for an identical receptor on the cell surface.