Health & Environmental Research Online (HERO)


Dibutyl Phthalate (DBP)

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1,427 References Were Found:

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Deproteinized natural rubber film forming polymeric solutions for nicotine transdermal delivery

Authors: Pichayakorn, W; Suksaeree, J; Boonme, P; Amnuaikit, T; Taweepreda, W; Ritthidej, GC (In Press) Pharm Dev Technol. HERO ID: 1332535

[Less] Film forming polymeric solutions were prepared from DNRL blended with MC, PVA, or SAG, together with . . . [More] Film forming polymeric solutions were prepared from DNRL blended with MC, PVA, or SAG, together with dibutylphthalate or glycerine used as plasticizers. These formulations were easily prepared by simple mixing. In a preliminary step, in situ films were prepared by solvent evaporation in a Petri-dish. Their mechanical and physicochemical properties were determined. The in vitro release and skin permeation of nicotine dissolved in these blended polymers were investigated by a modified Franz diffusion cell. The formulations had a white milky appearance, and were homogeneous and smooth in texture. Their pH was suitable for usage in skin contact. The mechanical property of in situ films depended on the ingredients but all compatible films were in an amorphous phase. The DNRL/PVA was shown to be the most suitable mixture to form completed films. The in vitro release and skin permeation studies demonstrated a biphasic release that provided an initial rapid release followed by a constant release rate that fitted the Higuchi's model. Nicotine loaded DNRL/PVA series were selected for the stability test for 3 months. These formulations needed to be kept at 4°C in tight fitting containers. In conclusion, film forming polymeric solutions could be developed for transdermal nicotine delivery systems.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Assessment of organochlorine pesticides and plasticisers in the Selangor River basin and possible pollution sources

Authors: Santhi, VA; Mustafa, AM (In Press) Environmental Monitoring and Assessment. HERO ID: 1332544

[Less] A study on the quality of water abstracted for potable use was conducted in the Selangor River basin . . . [More] A study on the quality of water abstracted for potable use was conducted in the Selangor River basin from November 2008 to July 2009. Seven sampling sites representing the intake points of water treatment plants in the basin were selected to determine the occurrence and level of 15 organochlorine pesticides (OCPs), six phthalate esters (PAEs) and bisphenol A (BPA). Results indicated OCPs were still detected regularly in 66.1 % of the samples with the Σ(15)OCPs ranging from 0.6-25.2 ng/L. The first data on PAEs contamination in the basin revealed Σ(6)PAEs concentrations were between 39.0 and 1,096.6 ng/L with a median concentration of 186.0 ng/L while BPA concentration ranged from <1.2 to 120.0 ng/L. Although di-n-butyl phthalate was detected in all the samples, concentrations of di-ethyl(hexyl)phthalate were higher. Sampling sites located downstream recorded the highest concentrations, together with samples collected during the dry season. Comparison of the detected contaminants with the Department of Environment Water Quality Index (DOE-WQI) showed some agreement between the concentration and the current classification of stream water. While the results suggest that the sites were only slightly polluted and suitable to be used as drinking water source, its presence is cause for concern especially to the fragile firefly "Pteroptyx tener" ecosystem located further downstream.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Occurrence and Profiles of Phthalates in Foodstuffs from China and Their Implications for Human Exposure

Authors: Guo, Y; Zhang, Z; Liu, L; Li, Y; Ren, N; Kannan, K (In Press) Journal of Agricultural and Food Chemistry. HERO ID: 1311703

[Less] Phthalate esters are used in a wide variety of consumer products, and human exposure to this class of . . . [More] Phthalate esters are used in a wide variety of consumer products, and human exposure to this class of compounds is widespread. Nevertheless, studies on dietary exposure of humans to phthalates are limited. In this study, nine phthalate esters were analyzed in eight categories of foodstuffs (n = 78) collected from Harbin and Shanghai, China, in 2011. Dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), diisobutyl phthalate (DIBP), benzyl butyl phthalate (BzBP), and diethylhexyl phthalate (DEHP) were frequently detected in food samples. DEHP was the major compound found in most of the food samples, with concentrations that ranged from below the limit of quantification (LOQ) to 762 ng/g wet weight (wt). The concentrations of phthalates in food samples from China were comparable to concentrations reported for several other countries, but the profiles were different; DMP was found more frequently in Chinese foods than in foods from other countries. The estimated daily dietary intake of phthalates (EDI(diet)) was calculated based on the concentrations measured and the daily ingestion rates of food items. The EDI(diet) values for DMP, DEP, DIBP, DBP, BzBP, and DEHP (based on mean concentrations) were 0.092, 0.051, 0.505, 0.703, 0.022, and 1.60 μg/kg-bw/d, respectively, for Chinese adults. The EDI(diet) values calculated for phthalates were below the reference doses suggested by the United States Environmental Protection Agency (EPA). Comparison of total daily intakes, reported previously based on a biomonitoring study, with the current dietary intake estimates suggests that diet is the main source of DEHP exposure in China. Nevertheless, diet accounted for only <10% of the total exposure to DMP, DEP, DBP, and DIBP, which suggested the existence of other sources of exposure to these phthalates.

Technical Report
Technical Report

Supplemental Boberg data for HERO ID 806135 from email communication

Author: Boberg, J (2016) Washington, DC: U.S. Environmental Protection Agency. [EPA Report] HERO ID: 3102218

[Less] This material represents the individual male reproductive data from the journal article (obtained via . . . [More] This material represents the individual male reproductive data from the journal article (obtained via email communication with Dr. Julie Boberg):

Boberg, J; Christiansen, S; Axelstad, M; et al. (2010) Reproductive and behavioral effects of diisononyl phthalate (DINP) in perinatally exposed rats. Reproductive Toxicology. 2011 Feb; 31(2):200-9. [HERO ID 806135]

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Expression patterns of DLK1 and INSL3 identify stages of Leydig cell differentiation during normal development and in testicular pathologies, including testicular cancer and Klinefelter syndrome

Authors: Lottrup, G; Nielsen, JE; Maroun, LL; Møller, LMA; Yassin, M; Leffers, H; Skakkebæk, NE; Rajpert-De Meyts, E (2014) Human Reproduction 29:1637-1650. HERO ID: 2509294

[Less] STUDY QUESTION What is the differentiation stage of human testicular interstitial cells, in particular . . . [More] STUDY QUESTION What is the differentiation stage of human testicular interstitial cells, in particular Leydig cells (LC), within micronodules found in patients with infertility, testicular cancer and Klinefelter syndrome? SUMMARY ANSWER The Leydig- and peritubular-cell populations in testes with dysgenesis contain an increased proportion of undifferentiated cells when compared with control samples, as demonstrated by increased delta-like homolog 1 (DLK1) and decreased insulin-like peptide 3 (INSL3) expression. WHAT IS KNOWN ALREADY Normal LC function is essential for male development and reproduction. Signs of LC failure, including LC micronodules, are often observed in patients with reproductive disorders. STUDY DESIGN, SIZE, PARTICIPANTS In this retrospective study, a panel of markers and factors linked to the differentiation of LCs was investigated in 33 fetal and prepubertal human specimens and in 58 adult testis samples from patients with testicular germ cell tumours, including precursor carcinoma in situ (CIS), infertility or Klinefelter syndrome. PARTICIPANTS/MATERIALS, SETTING, METHODS The expression patterns of DLK1, INSL3, chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII), cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1) and smooth muscle actin (SMA) were investigated by immunohistochemistry and quantitative RT–PCR. The percentage of positive LCs was estimated and correlated to total LC numbers and serum levels of reproductive hormones. MAIN RESULTS AND THE ROLE OF CHANCE DLK1, INSL3 and COUP-TFII expression changed during normal development and was linked to different stages of LC differentiation: DLK1 was expressed in all fetal LCs, but only in spindle-shaped progenitor cells and in a small subset of polygonal LCs in the normal adult testis; INSL3 was expressed in a subset of fetal LCs, but in the majority of adult LCs; and COUP-TFII was expressed in peritubular and mesenchymal stroma cells at all ages, in fetal LCs early in gestation and in a subset of adult LCs. CYP11A1 was expressed in the majority of LCs regardless of age and pathology and was the best general LC marker examined here. SMA was weakly expressed in peritubular cells in the fetal and infantile testis, but strongly expressed in the adult testis. In pathological testes, the numbers of DLK1-positive interstitial cells were increased. The proportion of DLK1-positive LCs correlated with total LC numbers (R = 0.53; P < 0.001) and was higher in testis with enlargement of the peritubular layers (P < 0.01), which was also highly associated with DLK1 expression in the peritubular compartment (P < 0.001). INSL3 expression was absent in some, but not all LC micronodules, and in the majority of LCs, it was mutually exclusive of DLK1. LIMITATIONS, REASONS FOR CAUTION The number of samples was relatively small and no true normal adult controls were available. True stereology was not used for LC counting, instead LCs were counted in three fields of 0.5 µm2 surface for each sample. WIDER IMPLICATIONS OF THE FINDINGS The population of LCs, especially those clustered in large nodules, are heterogeneous and comprise cells at different stages of differentiation. The study demonstrated that the differentiation and function of LCs, and possibly also peritubular cells, are impaired in adult men with testicular pathologies including testis cancer and Klinefelter syndrome. STUDY FUNDING/COMPETING INTEREST(S) This work was funded by Rigshospitalet's research funds, the Danish Cancer Society and Kirsten and Freddy Johansen's foundation. The authors have no conflicts of interest.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

The estrogen receptor signaling pathway activated by phthalates is linked with transforming growth factor-beta in the progression of LNCaP prostate cancer models

Authors: Lee, HR; Hwang, KA; Choi, KC (2014) International Journal of Oncology 45:595-602. HERO ID: 2509295

[Less] The distinct roles of estrogen receptors (ERs) related with androgen receptors (ARs) have been proposed . . . [More] The distinct roles of estrogen receptors (ERs) related with androgen receptors (ARs) have been proposed in prostate cancer, while the involvement of transforming growth factor-beta (TGF-beta) has been reported in the progression of prostate cancer. In this study, we examined whether the TGF-beta signaling pathway is associated with ER signaling in LNCaP prostate cancer cells, which express ER alpha, ER beta and ARs. We determined whether the exposure to phthalates may induce prostate cancer progression by affecting molecular crosstalk between ER and TGF-beta signaling pathways. Cell viability was measured in LNCaP cells by MTT assay following treatment with di-n-buthyl phthalate (DBP). RT-PCR and immunoblot assay were performed to examine the expression levels of cell cycle-related genes and the TGF-beta signaling cascade. A mouse xenograft model of prostate cancer was generated, and immunohistochemical and BrdU assay were carried out to determine the effect of DBP in this mouse model. DBP, a type of phthalate, was shown to promote LNCaP cell proliferation by upregulating the gene expression of c-myc and cyclin D1 and by downregulating the expression of p21. DBP significantly reduced the protein expression of p-smad similarly to E2. These regulations caused by DBP were reversed by ICI 182,780, an ER antagonist, indicating that DBP may affect crosstalk between TGF-beta and ER signals. In an in vivo mouse model, tumor volume of mice exposed to DBP was increased. Number of cells in S phase of cell cycle was increased by DBP, while expression of p21 protein was reduced in the tissues of DBP-treated mice. These results indicate that DBP may induce the growth of LNCaP prostate cancer by acting on the crosstalk between TGF-beta and ER signaling pathways.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Variations in Microbial Flora in Different Phases of Refuse in Response

Authors: Fang, C; Shen, D (2014) HERO ID: 2510979

[Less] Simulated municipal solid waste (MSW) based on the actual proportional characteristics of MSW was loaded . . . [More] Simulated municipal solid waste (MSW) based on the actual proportional characteristics of MSW was loaded into a simulated leachate recirculation bioreactor landfill and the abundance of common and tolerant microbes exposed to dibutyl phthalate (DBP) were investigated in the initial, acidic and methanogenic phases. The results showed that the abundance of bacteria was greatest, while that of actinomycetes was smallest. The growth of microorganisms was not significantly inhibited by DBP during the initial phase; however, the growth of actinomycetes and fungi was inhibited during both the acidic and methanogenic phases, and the inhibition of actinomycetes was greater than that of fungi. When the DBP concentration was 5.0 g l(-1), the inhibition ratio against actinomycetes was 89.5% and 80.9% in the refuse from the acidic phase and methanogenic phase, respectively, while it was 86.2% and 51.0%, respectively, against fungi during the same period. The toxic effects of DBP on microorganisms in refuse occurred in the order of actinomycetes > fungi > bacteria. In addition, the populations of bacteria, fungi and actinomycetes were significantly and positively correlated with dehydrogenase activity, but negatively correlated with the VSS and BDM of refuse (P<0.01).

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Isolation and Identification of Insecticidal Component from Roots of Stellera chamaejasme L. Against Locusta migratoria manilensis

Authors: Wu, L; Pu, Tao; Gao, M; Chen, L; Tao, Ke; Liu, Kun; Hou, T (2014) HERO ID: 2511089

[Less] In order to find the effective bio-pesticide to against locusts, we tested the bioactivity of the different . . . [More] In order to find the effective bio-pesticide to against locusts, we tested the bioactivity of the different extracts from roots of Stellera chamaejasme L., respectively. The results of bioassay indicated that petroleum ether extracts had obviously insecticidal activity towards locusts. With silica column chromatography method, we isolated one compound from petroleum ether extracts. Spectra data of (NMR)-N-1 and (NMR)-N-13 showed the insecticidal compound in the roots of Stellera chamaejasme. L against Locusta migratoria manilensis was dibutyl phthalate. The LC50 of dibutyl phthalate was found to be 292.68 ppm.

Book/Book Chapter
Book/ Chapter

Effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP

Authors: Li, H; Zhang, Yi; Wang, P; Yu, J (2014) In Li, H; Xu, Q; Ge, H (Eds.), Environmental engineering : selected, peer reviewed papers from the 3rd International Conference on Energy, Environment and Sustainable Development (EESD 2013), November 12-13, 2013, Shanghai, China (pp. 797-800). Zurich, Switzerland: Trans Tech Publications. HERO ID: 2509297

[Less] Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic . . . [More] Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic effects, is widely used in many industries such as plastics, pharmaceuticals, plastic food packaging materials. This article intends to study the effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP via vitro test. Testicular sertoli cells of rates were incubated by medium containing DBP in different concentrations (0 mu g/ml,1 mu g/ml,10 mu g/ml, 100 mu g/ml). after culturing for 24 hours the expression of vimentin was detected by immunofluorescence; the expression of vimentin mRNA detected by RT-PCR; Western blot analyzed the expression of related protein of JNK/MAPK pathways in sertoli cells. The results showed that vimentin highly express in sertoli cells and gradually decrease with the increase of poisonous doses. The mRNA expression of vimentin in each exposure group has a downtrend with the increase of does. The total protein of INK and P-INK protein increased gradually in a dose effect manner. These results suggested that DBP can decrease the expression of vimentin mRNA, the decrease of which was regulated by JNK/MAPK signal transduction pathway that activated by DBP, and then induced the apoptosis of Sertoli cell.

The "refereed" or "peer review" status of a journal comes from the Ulrichsweb Global Serials Directory (http://ulrichsweb.serialssolutions.com/), as supplied by the publisher. The term refers to the system of critical evaluation of manuscripts/articles by professional colleagues or peers. The content of refereed publications is sanctioned, vetted, or otherwise approved by a peer-review or editorial board. The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. Publications subject to the referee process are assumed, then, to contain higher quality content than those that are not.
Peer Reviewed Journal Article

Flow Injection Potentiometric Determination of Terazosin Hydrochloride Using Modified Carbon Paste Electrodes

Authors: Zayed, SIM; Khalil, MM; Issa, YM; Khalefa, HR (2014) HERO ID: 2346031

[Less] Three carbon paste electrodes for determination of terazosin hydrochloride (TerazCl) were prepared based . . . [More] Three carbon paste electrodes for determination of terazosin hydrochloride (TerazCl) were prepared based on ion association complexes with sodium tetraphenylborate (NaTPB), ammonium reineckate and phosphotungstic acid (PTA), using dibutyl phthalate as solvent mediator. The developed sensors showed a near-Nernstian response over the concentration range 3.98x10(-5)-1.00x10(-2) mol L-1 TerazCl with detection limits of 2.18x10(-5), 3.60x10(-5) and 1.26x10(-5) mol L-1 TerazCl, in case of tetraphenylborate, reineckate, and phosphotungstate, respectively. The proposed sensors exhibit good selectivity for terazosin with respect to a large number of inorganic cations, sugars, amino acids and vitamins. The sensors were successfully applied for the potentiometric determination of TerazCl in pharmaceutical preparation in batch and flow injection conditions. The sensors were also applied for the determination of TerazCl in spiked human urine samples by using the standard addition method.