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HERO ID
1015734
Reference Type
Journal Article
Title
Members of rice plasma membrane intrinsic proteins subfamily are involved in arsenite permeability and tolerance in plants
Author(s)
Mosa, KA; Kumar, K; Chhikara, S; Mcdermott, J; Liu, Z; Musante, C; White, JC; Dhankher, OP
Year
2012
Is Peer Reviewed?
1
Journal
Transgenic Research
ISSN:
0962-8819
Volume
21
Issue
6
Page Numbers
1265-1277
Language
English
PMID
22350764
DOI
10.1007/s11248-012-9600-8
Web of Science Id
WOS:000311496500011
URL
http://link.springer.com/10.1007/s11248-012-9600-8
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Abstract
Rice accumulates high level of arsenic (As) in its edible parts and thus plays an important role in the transfer of As into the food chain. However, the mechanisms of As uptake and its detoxification in rice are not well understood. Recently, members of the Nodulin 26-like intrinsic protein (NIP) subfamily of plant aquaporins were shown to transport arsenite in rice and Arabidopsis. Here we report that members of the rice plasma membrane intrinsic protein (PIP) subfamily are also involved in As tolerance and transport. Based on the homology search with the mammalian AQP9 and yeast Fps1 arsenite transporters, we identified and cloned five rice PIP gene subfamily members. qRT-PCR analysis of PIPs in rice root and shoot tissues revealed a significant down regulation of transcripts encoding OsPIP1;2, OsPIP1;3, OsPIP2;4, OsPIP2;6, and OsPIP2;7 in response to arsenite treatment. Heterologous expression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Xenopus laevis oocytes significantly increased the uptake of arsenite. Overexpression of OsPIP2;4, OsPIP2;6, and OsPIP2;7 in Arabidopsis yielded enhanced arsenite tolerance and higher biomass accumulation. Further, these transgenic plants showed no significant accumulation of As in shoot and root tissues in long term uptake assays. Whereas, short duration exposure to arsenite caused both active influx and efflux of As in the roots. The data suggests a bidirectional arsenite permeability of rice PIPs in plants. These rice PIPs genes will be highly useful for engineering important food and biofuel crops for enhanced crop productivity on contaminated soils without increasing the accumulation of toxic As in the biomass or edible tissues.
Keywords
Aquaporins; Arsenite transport; Rice; Plasma membrane intrinsic proteins; Arabidopsis; Xenopus laevis
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
ToxNet
WOS
Excluded
Non Peer Reviewed
2. Lit Search Updates through Oct 2015
WOS
Initial Filter
Non Peer Reviewed
3. Initial Filter through Oct 2015
Non Peer-Reviewed
7. Other Studies through Oct 2015
Ecology
•
Arsenic (Inorganic)
1. Literature
PubMed
Toxline, TSCATS, & DART
Lit search updates through Oct 2015
2. Initial Filter
Non peer-reviewed
3. Hazard ID Screening
Other potentially supporting studies
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