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HERO ID
1021907
Reference Type
Journal Article
Title
Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines
Author(s)
Mukherjee, SG; O'Claonadh, N; Casey, A; Chambers, G
Year
2012
Is Peer Reviewed?
1
Journal
Toxicology In Vitro
ISSN:
0887-2333
EISSN:
1879-3177
Volume
26
Issue
2
Page Numbers
238-251
Language
English
PMID
22198051
DOI
10.1016/j.tiv.2011.12.004
Web of Science Id
WOS:000301019600007
Abstract
In this study the cytotoxic effect of commercially available silver (Ag) nanoparticle was evaluated using human dermal and cervical cancer cell lines. Prior to the cellular studies a full particle size characterisation was carried out using Dynamic Light Scattering (DLS), Transmission Electron Microscopy and Scanning Electron Microscopy in distilled water and cell culture media. The Zeta Potential (ZP) associated with the Ag nanoparticle was also determined in order to assess its stability in the solutions and its possible interaction with the media. The DLS and ZP study have suggested interaction of Ag nanoparticles with the media, which can lead to secondary toxicity. The toxic effects of Ag nanoparticles were then evaluated using different cytotoxic endpoints namely the lysosomal activity, mitochondrial metabolism, basic cellular metabolism, cellular protein content and cellular proliferative capacity. The cytotoxic effect of Ag nanoparticle was dependant on dose, exposure time and on the cell line tested. Further investigation was carried out on HeLa and HaCaT cell lines to elucidate the mechanism of its cytotoxicity. The Ag nanoparticle was noted to induce elevated levels of oxidative stress, glutathione depletion and damage to the cell membrane as found from the adenylate kinase assay and that leads to the apoptosis. Overall, significant differences were observed between the sensitivity of the two cell lines which can be understood in terms of their natural antioxidant levels.
Keywords
Physical characterization; Cytotoxicity assessment; Mitochondrial metabolism; Alamar Blue assay; Coomassie Blue assay; Lysosomal activity; Cellular proliferative capacity; LD50; ROS; Glutathione; Adenylate kinase; Apoptosis; Comparative cytotoxicity
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Nanoscale Silver
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