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1021908 
Journal Article 
Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells 
Asare, N; Instanes, C; Sandberg, WJ; Refsnes, M; Schwarze, P; Kruszewski, M; Brunborg, G 
2012 
1 
Toxicology
ISSN: 0300-483X
EISSN: 1879-3185 
291 
1-3 
65-72 
English 
22085606 
WOS:000300752800009 
Serious concerns have been expressed about potential risks of engineered nanoparticles. Regulatory health risk assessment of such particles has become mandatory for the safe use of nanomaterials in consumer products and medicines; including the potential effects on reproduction and fertility, are relevant for this risk evaluation. In this study, we examined effects of silver particles of nano- (20nm) and submicron- (200nm) size, and titanium dioxide nanoparticles (TiO(2)-NPs; 21nm), with emphasis on reproductive cellular- and genotoxicity. Ntera2 (NT2, human testicular embryonic carcinoma cell line), and primary testicular cells from C57BL6 mice of wild type (WT) and 8-oxoguanine DNA glycosylase knock-out (KO, mOgg1(-/-)) genotype were exposed to the particles. The latter mimics the repair status of human testicular cells vs oxidative damage and is thus a suitable model for human male reproductive toxicity studies. The results suggest that silver nano- and submicron-particles (AgNPs) are more cytotoxic and cytostatic compared to TiO(2)-NPs, causing apoptosis, necrosis and decreased proliferation in a concentration- and time-dependent manner. The 200nm AgNPs in particular appeared to cause a concentration-dependent increase in DNA-strand breaks in NT2 cells, whereas the latter response did not seem to occur with respect to oxidative purine base damage analysed with any of the particles tested. 
Titanium dioxide nanoparticles; Cellular metabolic activity; Cell viability; DNA damage; Comet assay 
Other
• Nanoscale Silver