Health & Environmental Research Online (HERO)


Print Feedback Export to File
1037853 
Journal Article 
Simultaneous determination of tyrosine, tryptophan and 5-hydroxytryptamine in serum of MDD patients by high performance liquid chromatography with fluorescence detection 
Sa, M; Ying, L; Tang, AG; Xiao, LD; Ren, YP 
2012 
Yes 
Clinica Chimica Acta
ISSN: 0009-8981
EISSN: 1873-3492 
413 
11-12 
973-977 
English 
22402312 
BACKGROUND: Tyrosine (Tyr), Tryptophan (Trp) and 5-hydroxytryptamine (5-HT) are important amino acids in vivo and have been hypothesized to be involved in many mental disorders. We developed a rapid and sensitive HPLC method for simultaneous measurement of serum Tyr, Trp and 5-HT and explored the clinical significances of Tyr, Trp and 5-HT and the 5-HT/Trp ratio for patients with major depressive disorder (MDD) disease. METHODS: Serum samples were deproteinized by 5% perchloric acid and separated on an Atlantis C18 column (4.6×150mm, 5μm) with the mobile phase consisting of 0.1mol/l KH(2)PO(4) and methanol (85:15, V/V).The eluates were monitored by the fluorescence detection with programmed wavelength. RESULTS: Analysis was achieved in <12.0min. The limits of quantification were 0.014, 0.005, and 0.024μmol/l for Tyr, Trp and 5-HT, respectively. Reproducibility and recovery were satisfactory. Tyr, Trp and 5-HT and the 5-HT/Trp ratio were significantly decreased in patients with MDD. CONCLUSIONS: In diseases, like MDD, Tyr, Trp and 5-HT play an important role. This method can potentially be applied as prognostic or diagnostic tool or even to follow the evolution of the illness or of the treatment. 
High performance liquid chromatography; Fluorescence detection; Tyrosine; Tryptophan; 5-hydroxytryptamine; Major depressive disorder 
IRIS
• Methanol (Non-Cancer)
     Search Jan 30 2013
          WOS