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Citation
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HERO ID
1037880
Reference Type
Journal Article
Title
Extinction coefficient for red-shifted chlorophylls: Chlorophyll d and chlorophyll f
Author(s)
Li, Y; Scales, N; Blankenship, RE; Willows, RD; Chen, M
Year
2012
Is Peer Reviewed?
1
Journal
Biochimica et Biophysica Acta
ISSN:
0006-3002
EISSN:
1878-2434
Volume
1817
Issue
8
Page Numbers
1292-1298
Language
English
PMID
22395150
DOI
10.1016/j.bbabio.2012.02.026
Web of Science Id
WOS:000306202700022
Abstract
Both chlorophyll f and chlorophyll d are red-shifted chlorophylls in oxygenic photosynthetic organisms, which extend photon absorbance into the near infrared region. This expands the range of light that can be used to drive photosynthesis. Quantitative determination of chlorophylls is a crucial step in the investigation of chlorophyll-photosynthetic reactions in the field of photobiology and photochemistry. No methods have yet been worked out for the quantitative determination of chlorophyll f. There is also no method available for the precise quantitative determination of chlorophyll d although it was discovered in 1943. In order to obtain the extinction coefficients (ε) of chlorophyll f and chlorophyll d, the concentrations of chlorophylls were determined by Inductive Coupled Plasma Mass Spectrometry according to the fact that each chlorophyll molecule contains one magnesium (Mg) atom. Molar extinction coefficient ε(chl f) is 71.11×10(3)Lmol(-1)A(707nm)cm(-1) and ε(chl d) is 63.68×10(3)Lmol(-1)A(697nm)cm(-1) in 100% methanol. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: From Natural to Artificial Photosynthesis.
Keywords
Chlorophyll; Red-shifted chlorophyll; Chlorophyll f; Chlorophyll d; Extinction coefficient; Spectrophotometric equation
Tags
IRIS
•
Methanol (Non-Cancer)
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