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Citation
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HERO ID
1038264
Reference Type
Journal Article
Title
Comparative transcriptomics and proteomics analysis of citrus fruit, to improve understanding of the effect of low temperature on maintaining fruit quality during lengthy post-harvest storage
Author(s)
Yun, Z; Jin, S; Ding, Y; Wang, Z; Gao, H; Pan, Z; Xu, J; Cheng, Y; Deng, X
Year
2012
Is Peer Reviewed?
Yes
Journal
Journal of Experimental Botany
ISSN:
0022-0957
EISSN:
1460-2431
Volume
63
Issue
8
Page Numbers
2873-2893
Language
English
PMID
22323274
DOI
10.1093/jxb/err390
Web of Science Id
WOS:000304196900004
URL
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/err390
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Abstract
Fruit quality is a very complex trait that is affected by both genetic and non-genetic factors. Generally, low temperature (LT) is used to delay fruit senescence and maintain fruit quality during post-harvest storage but the molecular mechanisms involved are poorly understood. Hirado Buntan Pummelo (HBP; Citrus grandis × C. paradis) fruit were chosen to explore the mechanisms that maintain citrus fruit quality during lengthy LT storage using transcriptome and proteome studies based on digital gene expression (DGE) profiling and two-dimensional gel electrophoresis (2-DE), respectively. Results showed that LT up-regulated stress-responsive genes, arrested signal transduction, and inhibited primary metabolism, secondary metabolism and the transportation of metabolites. Calcineurin B-like protein (CBL)-CBL-interacting protein kinase complexes might be involved in the signal transduction of LT stress, and fruit quality is likely to be regulated by sugar-mediated auxin and abscisic acid (ABA) signalling. Furthermore, ABA was specific to the regulation of citrus fruit senescence and was not involved in the LT stress response. In addition, the accumulation of limonin, nomilin, methanol, and aldehyde, together with the up-regulated heat shock proteins, COR15, and cold response-related genes, provided a comprehensive proteomics and transcriptomics view on the coordination of fruit LT stress responses.
Keywords
fruit quality; low-temperature storage; metabolism and metabolite transportation; proteomics; senescence; signal transduction; transcriptomics
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Methanol (Non-Cancer)
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