US EPA

1044186 

Journal Article 

Recovery of carotenoids from shrimp waste in organic solvents 

Sachindra, NM; Bhaskar, N; Mahendrakar, NS 

2006 

1 

Waste Management
ISSN: 0956-053X
EISSN: 1879-2456 

26 

10 

1092-1098 

English 

16129592 

10.1016/j.wasman.2005.07.002 

WOS:000240845000004 

Shrimp waste, which is produced in large quantities in the Indian seafood processing industries, is one of the important sources of natural carotenoids. Studies were carried out to assess the extractability of shrimp waste carotenoids in different organic solvents and solvent mixtures and to optimize the extraction conditions for maximum yield. A 50:50 mixture of isopropyl alcohol and hexane gave the highest (43.9 microg/g waste) carotenoid extraction yield compared to acetone, methanol, ethanol, isopropyl alcohol, ethyl acetate, ethyl methyl ketone, petroleum ether, and hexane individually and to a mixture of acetone and hexane. Extraction conditions such as percentage of hexane in the solvent mixture of isopropyl alcohol and hexane, ratio of solvent to waste and number of extractions was optimized using a statistically designed experiment. The optimized conditions for maximum yield of carotenoids were 60% hexane in solvent mixture, solvent mixture to waste ratio of 5:1 in each extraction and three extractions. A regression equation for predicting the carotenoid yield as a function of three processing variable (hexane % in solvent mixture, solvent-to-waste ratio and number of extractions) was derived by statistical analysis, and a model with predictive ability of 0.98 was obtained.