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Citation
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HERO ID
1047045
Reference Type
Journal Article
Title
Validation of a screening method based on liquid chromatography coupled to high-resolution mass spectrometry for analysis of perfluoroalkylated substances in biota
Author(s)
Berger, U; Haukås, M
Year
2005
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography A
ISSN:
0021-9673
EISSN:
1873-3778
Volume
1081
Issue
2
Page Numbers
210-217
Language
English
PMID
16038211
DOI
10.1016/j.chroma.2005.05.064
Web of Science Id
WOS:000230320900011
URL
http://www.sciencedirect.com/science/article/pii/S0021967305010848
Exit
Abstract
A screening method for analysis of perfluoroalkylated substances (PFAS) in biota samples has been developed and validated using liver samples from polar cod (Boreogadus saida) and glaucous gull (Larus hyperboreus). The method was based on extraction of target compounds from homogenised samples into the solvent mixture used as mobile phase in high-performance liquid chromatography (HPLC), i.e. methanol/water (50:50; 2 mM ammonium acetate). The extract was filtered and directly injected into a HPLC/time-of-flight mass spectrometry (TOF-MS) system. Quantification was performed using 7H-perfluoroheptanoic acid as internal standard and a calibration standard solution dissolved in sample extract for each matrix type (matrix-matched calibration standard). The method is very time and cost efficient. Except for long-chain compounds and perfluorooctane sulfonamide (which cannot be covered by this method), recoveries were between 60% and 115% and method detection limits were in the range 0.04-1.3 ng/g wet weight. Blank values could be neglected with the exception of perfluorooctane sulfonate (PFOS), perfluorohexanoic acid (PFHxA) and perfluorooctanoic acid (PFOA). One of the major challenges in PFAS analysis is ionisation disturbance by co-eluting matrix in the ion source of the mass spectrometer. Both matrix and analyte specific signal enhancement and suppression was observed and quantified. Repeated extractions (n = 3) gave relative standard deviations (RSD) <35% for all PFAS. Accuracy was examined by comparing the screening method to the generally applied ion pair extraction (IPE) method. PFAS concentration values of a glaucous gull liver sample deviated by less than 30% for the two methods, provided that matrix-matched calibration standards were employed in both methods.
Keywords
perfluoroalkylated substances; perfluorooctane sulfonate; PFOS; high-resolution MS; method validation; screening method; liver samples
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
PubMed
Web of Science
Not prioritized for screening
7H-Perfluoroheptanoic acid
Perfluorooctane
•
^Per- and Polyfluoroalkyl Substances (PFAS)
PFHpA (375-85-9)
Literature Search
Pubmed
WOS
PFHxA (307-24-4)
Literature Search
Pubmed
WOS
PFOSA (754-91-6)
Literature Search
WOS
•
PFAS 150
Literature Search Update December 2020
PubMed
WOS
Literature Search August 2019
PubMed
Web of Science
Not prioritized for screening
Ammonium perfluorooctanoate
Perfluoroheptanoic acid
Perfluorohexanoic acid
Perfluorooctane
Perfluorooctanesulfonic acid
Perfluorooctanoic acid
1,1,1,3,3,3-Hexafluoro-2- (fluoromethoxy)propane
•
PFBA
Protocol References
•
PFHpA
Literature Search
Pubmed
WOS
Screening Results
Excluded/Not on Topic
•
PFHxA
Literature Search
Pubmed
WOS
Literature Search
Pubmed
WOS
Scopus: April 2021
Screening Results
Excluded/Not on Topic
Title and Abstract Screening
Excluded
Not relevant to PECO
HAWC
•
PFOSA
Literature Search
Pubmed
WOS
Screening Results
Excluded/Not on Topic
•
Yale PFAS Liver study
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