US EPA

1070460 

Journal Article 

Plant proteins, minerals and trace elements of Eurycoma longifolia (Tongkat Ali) 

Chua, LS; Abdul-Rahman, N; Rosidi, B; Lee, CT 

2013 

1 

Natural Product Research
ISSN: 1478-6419
EISSN: 1478-6427 

27 

4-5 

314-318 

English 

22468741 

10.1080/14786419.2012.676552 

WOS:000315793600004 

A water extraction method has been used to extract plant proteins from the roots of Eurycoma longifolia harvested from Perak and Pahang, Malaysia. On the basis of the spectroscopic Bradford assay, Tongkat Ali Perak and Pahang contained 0.3868 and 0.9573 mg mL-1 of crude protein, respectively. The crude proteins were separated by one dimensional 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis into two (49.8 and 5.5 kD) and four (49.8, 24.7, 21.1 and 5.5 kD) protein spots for Tongkat Ali Perak and Pahang, respectively. Isoleucine was present in the highest concentration significantly. Both plant samples showed differences in the mineral and trace element profiles, but the minerals calcium, magnesium and potassium were present in the highest concentration. The highly concerned toxic metals such as arsenic and lead were not detected. 

Eurycoma longifolia; SDS-PAGE; plant protein; amino acids; metals