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HERO ID
1070886
Reference Type
Journal Article
Title
Biofilm formation and microbial activity in a biofilter system in the presence of MTBE, ETBE and TAME
Author(s)
Purswani, J; Juárez, B; Rodelas, B; Gónzalez-López, J; Pozo, C
Year
2011
Is Peer Reviewed?
Yes
Journal
Chemosphere
ISSN:
0045-6535
EISSN:
1879-1298
Volume
85
Issue
4
Page Numbers
616-624
Language
English
PMID
21774959
DOI
10.1016/j.chemosphere.2011.06.106
Web of Science Id
WOS:000297662000011
Abstract
Emerging water contaminants derived from unleaded gasoline such as methyl tert-butyl ether (MTBE), ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME), are in need of effective bioremediation technologies for restoring water resources. In order to design the conditions of a future groundwater bioremediating biofilter, this work assesses the potential use of Acinetobacter calcoaceticus M10, Rhodococcus ruber E10 and Gordonia amicalis T3 for the removal of MTBE, ETBE and TAME in consortia or as individual strains. Biofilm formation on an inert polyethylene support material was assessed with scanning electron microscopy, and consortia were also analysed with fluorescent in situ hybridisation to examine the relation between the strains. A. calcoaceticus M10 was the best coloniser, followed by G. amicalis T3, however, biofilm formation of pair consortia favoured consortium M10-E10 both in formation and activity. However, degradation batch studies determined that neither consortium exhibited higher degradation than individual strain degradation. The physiological state of the three strains was also determined through flow cytometry using propidium iodide and 3'-dihexylocarbocyanine iodide thus gathering information on their viability and activity with the three oxygenates since previous microbial counts revealed slow growth. Strain E10 was observed to have the highest physiological activity in the presence of MTBE, and strain M10 activity with TAME was only maintained for 24 h, thus we believe that biotransformation of MTBE occurs within the active periods established by the cytometry analyses. Viable cell counts and oxygenate removal were determined in the presence of the metabolites tert-butyl alcohol (TBA) and tert-amyl alcohol (TAA), resulting in TBA biotransformation by M10 and E10, and TAA by M10. Our results show that A. calcoaceticus M10 and the consortium M10-E10 could be adequate inocula in MTBE and TAME bioremediating technologies.
Keywords
MTBE; ETBE; TAME; Biofilter; Biofilm; Biodegradation
Tags
IRIS
•
tert-Amyl ethyl ether (TAEE)
Initial Litsearch 6/2018
Pubmed
From PPRTV
•
tert-Amyl methyl ether (TAME)
Initial Litsearch 6/2018
Pubmed
Toxline
WOS
•
tert-Butanol
Considered Studies
Electronic Search
Excluded/ Not on Topic
Other Chemical/Non-tert-butanol
•
ETBE
Database Searches
Combined Dataset (After duplicates removed electronically)
Excluded / Not on Topic
Biodegradation/environmental fate
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