Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1071837
Reference Type
Journal Article
Title
Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems
Author(s)
Kato, T; Totsuka, Y; Ishino, K; Matsumoto, Y; Tada, Y; Nakae, D; Goto, S; Masuda, S; Ogo, S; Kawanishi, M; Yagi, T; Matsuda, T; Watanabe, M; Wakabayashi, K
Year
2013
Is Peer Reviewed?
1
Journal
Nanotoxicology
ISSN:
1743-5390
EISSN:
1743-5404
Volume
7
Issue
4
Page Numbers
452-461
Language
English
PMID
22397533
DOI
10.3109/17435390.2012.674571
Web of Science Id
WOS:000318590400009
Abstract
Abstract The genotoxic effects of multi-walled carbon nanotubes (MWCNTs) were examined by using in vitro and in vivo assays. MWCNTs significantly induced micronuclei in A549 cells and enhanced the frequency of sister chromatid exchange (SCE) in CHO AA8 cells. When ICR mice were intratracheally instilled with a single dose (0.05 or 0.2 mg/animal) of MWCNTs, DNA damage of the lungs, analysed by comet assay, increased in a dose-dependent manner. Moreover, DNA oxidative damage, indicated by 8-oxo-7,8-dihydro-2'-deoxyguanosine and heptanone etheno-deoxyribonucleosides, occurred in the lungs of MWCNT-exposed mice. The gpt mutation frequencies significantly increased in the lungs of MWCNT-treated gpt delta transgenic mice. Transversions were predominant, and G:C to C:G was clearly increased by MWCNTs. Moreover, many regions immunohistochemically stained for inducible NO synthase and nitrotyrosine were observed in the lungs of MWCNT-exposed mice. Overall, MWCNTs were shown to be genotoxic both in in vitro and in vivo tests; the mechanisms probably involve oxidative stress and inflammatory responses.
Keywords
Micronuclei; sister chromatid exchange; DNA damage; gpt mutation; oxidative stress
Tags
Other
•
Nanoscale Carbon
All References Cited
External Review Draft
Impacts
Analytical Methods
Peer Reviewed Draft
Impacts
Priority Area: Ch. 5 and Appendix F
Final Case Study
Impacts
Priority Area: Ch. 5 and Appendix F
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity