US EPA

1108954 

Journal Article 

Phenolic compounds and antioxidant activities of Rumex hastatus D. Don. leaves 

Sahreen, S; Khan, MR; Khan, RA 

2011 

Yes 

Journal of Medicinal Plant Research
ISSN: 1996-0875 

5 

13 

2755-2765 

English 

WOS:000297460000024 

In this study different solvent fractions (n-hexane, ethyl acetate, chloroform, butanol and aqueous) of the methanol extract of Rumex hastatus(RH) leaves were evaluated for antioxidant activities. The ethyl acetate fraction possessed high amount of total polyphenolics (106.3 +/- 3.93 mg GAE/g extract) while butanolic fraction was found with high flavonoid contents (59.8 +/- 1.94 mg RTE/g extract) as compared to other solvent fractions. Butanol fraction exhibited most promising DPPH (EC(50) 140 +/- 0.99 mu g/ml), iron chelation (EC(50) 70 +/- 2.1 mu g/ml) and for phosphomolybdate (EC(50) 31 +/- 1.25 mu g/ml) scavenging activity. However, ethyl acetate fraction exhibited the highest scavenging potential for super oxide radicals (EC(50) 40 +/- 0.78 mu g/ml), ABTS radicals (EC(50) 70 +/- 2.46 mu g/ml), hydroxyl radicals (EC(50) 9 +/- 0.56 mu g/ml) and inhibition of beta-carotene linoleic acid peroxidation (EC(50) 54 +/- 5.67 mu g/ml). Chloroform fraction showed the most potent antioxidant activities in scavenging of hydrogen peroxide (EC(50) 48 +/- 4.89 mu g/ml). The high correlation coefficient was found between EC(50) values of DPPH, superoxides, hydroxyl, hydrogen peroxide and ABTS radicals with total polyphenolic and flavonoids. HPLC studies of ethyl acetate fraction indicated the presence of luteolin (24.67 +/- 2.90 mg/g extract), kaempherol (17.03 +/- 1.67 mg/g extract), luteolin-7-O-glucoside (14.73 +/- 2.17 mg/g extract) and rutin (8.24 +/- 1.43 mg/g extract). Therefore, our study substantiated that RH leaves can be used as a good source of potential antioxidant or functional food material due to the presence of sufficient amount of phenolics such as luteolin and kaempherol. 

Rumex hastatus; antioxidant activity; flavonoids; phenolics; 2,2-diphenyl-1-picrylhydrazyl