Health & Environmental Research Online (HERO)


Print Feedback Export to File
1248942 
Journal Article 
Influence of age on arsenic-induced oxidative stress in rat 
Jain, A; Flora, GJ; Bhargava, R; Flora, SJ 
In Press 
Yes 
Biological Trace Element Research
ISSN: 0163-4984
EISSN: 1559-0720 
149 
382-390 
English 
Influence of age on arsenic-induced (0.05, 0.1, and 0.2 lethal dose to 50 % population (LD(50)) given intraperitoneally) oxidative stress was investigated in young, adult, and old rats at days 7 and 14 post-exposure. A significant dose-dependent effect of arsenic on biochemical variables suggestive of oxidative stress was noted at day 7 following exposure in old rats. The parameters which were significantly altered include an increased reactive oxygen species, thiobarbituric acid reactive substances (TBARS), catalase activity accompanied by a decreased glutathione level. At day 14 following arsenic exposure (0.05 and 0.1 LD(50) dose), we observed a significant oxidative injury as evident from significant depletion of superoxide dismutase (SOD) and catalase activities in blood and tissues in addition to more pronounced accumulation of arsenic in blood and tissues. Interestingly, the toxicity was pronounced in young and old rats compared with adult rats. Accumulation of arsenic found to be more prominent in old rats compared with young and adult, which might be due to impaired metabolism with ageing. We conclude that young and old animals are more vulnerable to the arsenic-induced oxidative injury which is comparable with arsenic accumulation in blood and tissues and duration of exposure. 
Arsenic; Oxidative stress; Glutathione; Age dependent 
• Arsenic Hazard ID
     1. Initial Lit Search
          PubMed
          ToxNet
          WOS
          Considered New
     2. Lit Search Updates through Oct 2015
          WOS
          Considered
     4. Considered through Oct 2015
     5. Additions through Oct 2015
     6. Cluster Filter through Oct 2015
     7. Other Studies through Oct 2015
          MOA
          iAs MOA Literature Categorization
               Cytotoxicity and Regenerative Proliferation
               Epigenetic Mechanisms
               Immune
          Susceptibility Category
               Lifestages
          Health Effect Category
               Not Relevant
• Arsenic (Inorganic)
     1. Literature
          PubMed
          Toxline, TSCATS, & DART
          Lit search updates through Oct 2015
     3. Hazard ID Screening
          Other potentially supporting studies
     4. Adverse Outcome Pathways/Networks Screening
          Relevant
     5. Susceptibility Screening
          Relevant
               Animal
• Arsenic MOA
     4. Adverse Outcome Pathways
          Oxidative stress related effects (includes non-specific SH reactions)
     5. Health Effect
          Hematology, Hematopoietic System
     1. MOA Literature Screening
          Health Effect Screening
          MOA Cluster
• Arsenic Susceptibility
     4. Susceptibility and Lifestages
          Lifestages
     5. Health Effect
          Not Relevant
     1. Susceptibility Literature Screening
          Keyword Search
     Life Stages Citation Mapping
          10%-15%