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Citation
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HERO ID
1250968
Reference Type
Technical Report
Title
GFAJ-1 Is an arsenate-resistant, phosphate-dependent organism
Author(s)
Erb, TJ; Kiefer, P; Hattendorf, B; Günther, D; Vorholt, JA
Year
2012
Is Peer Reviewed?
1
Journal
Science
ISSN:
0036-8075
EISSN:
1095-9203
Volume
337
Issue
6093
Page Numbers
467-470
Language
English
PMID
22773139
DOI
10.1126/science.1218455
Web of Science Id
WOS:000306802300043
URL
https://www.sciencemag.org/lookup/doi/10.1126/science.1218455
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Abstract
The bacterial isolate GFAJ-1 has recently been proposed to substitute arsenic for phosphorus to sustain growth. We have shown that GFAJ-1 is able to grow at low phosphate concentrations (1.7 μM), even in the presence of high concentrations of arsenate (40 mM), but lacked the ability to grow in phosphorus-depleted (<0.3 μM), arsenate-containing medium. High-resolution mass spectrometry analyses revealed that phosphorylated central metabolites and phosphorylated nucleic acids predominated. A few arsenylated compounds, including C6 sugar arsenates, were detected in extracts of GFAJ-1, when GFAJ-1 was incubated with AsO(4)(3-), but further experiments showed that they formed abiotically. Inductively coupled plasma mass spectrometry confirmed the presence of phosphorus and the absence of arsenic in nucleic acid extracts. Taken together, we conclude that GFAJ-1 is an arsenate-resistant, but still a phosphate-dependent bacterium.
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
WOS
ToxNet
WOS
Considered New
2. Lit Search Updates through Oct 2015
WOS
Considered
4. Considered through Oct 2015
6. Cluster Filter through Oct 2015
7. Other Studies through Oct 2015
Other
•
Arsenic (Inorganic)
1. Literature
PubMed
Toxline, TSCATS, & DART
Web of Science
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
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