Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1298114
Reference Type
Journal Article
Title
Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells
Author(s)
Vanparys, C; Maras, M; Lenjou, M; Robbens, J; Van Bockstaele, D; Blust, R; De Coen, W
Year
2006
Is Peer Reviewed?
1
Journal
Toxicology In Vitro
ISSN:
0887-2333
EISSN:
1879-3177
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Location
OXFORD
Volume
20
Issue
7
Page Numbers
1238-1248
Language
English
PMID
16797915
DOI
10.1016/j.tiv.2006.05.002
Web of Science Id
WOS:000241218900021
Abstract
The quantitative measurement of individual cells and their characteristics by means of flow cytometry is already for many years of great value for clinical studies. However, its potential as a tool in (eco)toxicology has only recently been discovered. Analysis of cell cycle kinetics with DNA-staining dyes can offer a valuable alternative to detect effects of chemicals on cell proliferation, an important endpoint in screening estrogen-like properties of chemicals. In the present study, flow cytometric cell cycle analysis in growth arrested MCF-7 cells exposed to five xenoestrogens correspond well with cell proliferation results of the conventionally used E-screen assay. Moreover, re-induction of proliferation in MCF-7 cells, indicated by the percentage of cells in S(ynthesis)-phase, is most pronounced after 24 h exposure, thus allowing a faster screening of xenoestrogens. This flow cytometric proliferation assay confirms that the estrogenic activity of structurally analogous parabens is mediated by the estrogen receptor pathway and is proportional to the alkyl chain length. Moreover, the ER-mediated mode of action of two fluorotelomer alcohols (6:2 FTOH and 8:2 FTOH), recently reported as xenoestrogenic, could be elucidated. These results support the potential of flow cytometric cell cycle kinetics as a screening assay for estrogen-like properties of chemicals.
Keywords
cell cycle analysis; flow cytometry; xenoestrogens; MCF-7
Tags
•
Additional PFAS (formerly XAgency)
•
FtOH 6:2
Screening Results
In vitro/ex vivo/in silico
Endocrine disruption
•
FtOH 8:2
Literature Search
Pubmed
WOS
Screening Results
In vitro/ex vivo/in silico
Endocrine disruption
•
^Per- and Polyfluoroalkyl Substances (PFAS)
FtOH 8:2 (678-39-7)
Literature Search
Pubmed
WOS
FtOH 6:2 (647-42-7)
Literature Search
Pubmed
WOS
•
PFAS 150
Literature Search August 2019
PubMed
Web of Science
Screened Studies
Supplemental
6:2 Fluorotelomer alcohol
8:2 Fluorotelomer alcohol
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity