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Citation
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HERO ID
1325395
Reference Type
Journal Article
Title
Widespread distribution of a lexA-regulated DNA damage-inducible multiple gene cassette in the Proteobacteria phylum
Author(s)
Abella, M; Erill, I; Jara, M; Mazón, G; Campoy, S; Barbé, J
Year
2004
Is Peer Reviewed?
1
Journal
Molecular Microbiology
ISSN:
0950-382X
Publisher
WILEY
Location
HOBOKEN
Volume
54
Issue
1
Page Numbers
212-222
Language
English
PMID
15458417
DOI
10.1111/j.1365-2958.2004.04260.x
Web of Science Id
WOS:000224178900018
URL
http://doi.wiley.com/10.1111/j.1365-2958.2004.04260.x
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Abstract
The SOS response comprises a set of cellular functions aimed at preserving bacterial cell viability in front of DNA injuries. The SOS network, negatively regulated by the LexA protein, is found in many bacterial species that have not suffered major reductions in their gene contents, but presents distinctly divergent LexA-binding sites across the Bacteria domain. In this article, we report the identification and characterization of an imported multiple gene cassette in the Gamma Proteobacterium Pseudomonas putida that encodes a LexA protein, an inhibitor of cell division (SulA), an error-prone polymerase (DinP) and the alpha subunit of DNA polymerase III (DnaE). We also demonstrate that these genes constitute a DNA damage-inducible operon that is regulated by its own encoded LexA protein, and we establish that the latter is a direct derivative of the Gram-positive LexA protein. In addition, in silico analyses reveal that this multiple gene cassette is also present in many Proteobacteria families, and that both its gene content and LexA-binding sequence have evolved over time, ultimately giving rise to the lexA lineage of extant Gamma Proteobacteria.
Keywords
dinp protein; DNA directed DNA polymerase gamma; LexA protein; protein; sula protein; unclassified drug; alpha chain; article; bacterial cell; binding site; cell division; cell function; cell viability; computer model; DNA damage; DNA repair; gene cassette; Gram negative bacterium; priority journal; Pseudomonas putida; Bacterial Proteins; Base Sequence; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Phylogeny; Proteobacteria; Serine Endopeptidases; SOS Response (Genetics); Bacteria (microorganisms); Gammaproteobacteria; Negibacteria; Posibacteria; Pseudomonas; uncultured gamma proteobacterium
Tags
IRIS
•
Diisononyl Phthalate (DINP)
Literature Search
LitSearch May 2013
Web of Science
Excluded: No Primary Data on Health Effects
Not chemical specific
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