Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
5490923
Reference Type
Journal Article
Title
N-butyl benzyl phthalate promotes breast cancer progression by inducing expression of lymphoid enhancer factor 1
Author(s)
Hsieh, TH; Tsai, CF; Hsu, CY; Kuo, PL; Hsi, E; Suen, JL; Hung, CH; Lee, JN; Chai, CY; Wang, SC; Tsai, EM
Year
2012
Is Peer Reviewed?
1
Journal
PLoS ONE
EISSN:
1932-6203
Volume
7
Issue
8
Page Numbers
e42750
Language
English
PMID
22905168
DOI
10.1371/journal.pone.0042750
Web of Science Id
WOS:000307331100061
Abstract
Environmental hormones play important roles in regulating the expression of genes involved in cell proliferation, drug resistance, and breast cancer risk; however, their precise role in human breast cancer cells during cancer progression remains unclear. To elucidate the effect of the most widely used industrial phthalate, n-butyl benzyl phthalate (BBP), on cancer progression, we evaluated the results of BBP treatment using a whole human genome cDNA microarray and MetaCore software and selected candidate genes whose expression was changed by more than ten-fold by BBP compared with controls to analyze the signaling pathways in human breast cancer initiating cells (R2d). A total of 473 genes were upregulated, and 468 were downregulated. Most of these genes are involved in proliferation, epithelial-mesenchymal transition, and angiogenesis signaling. BBP induced the viability, invasion and migration, and tube formation in vitro, and Matrigel plug angiogenesis in vivo of R2d and MCF-7. Furthermore, the viability and invasion and migration of these cell lines following BBP treatment was reduced by transfection with a small interfering RNA targeting the mRNA for lymphoid enhancer-binding factor 1; notably, the altered expression of this gene consistently differentiated tumors expressing genes involved in proliferation, epithelial-mesenchymal transition, and angiogenesis. These findings contribute to our understanding of the molecular impact of the environmental hormone BBP and suggest possible strategies for preventing and treating human breast cancer.
Tags
IRIS
•
BBP (Butyl benzyl phthalate)
Literature Search
LitSearch Dec 2012
PubMed
WOS
Studies with Supporting Data
Mechanistic and genotoxicity studies
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
WOS
Toxnet
Excluded
Source – no date limit through June 2013 (Private)
Pubmed
WOS
ToxNet
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity