Shao, M; Chen, YH; Li, XY
A method was developed for the consecutive online separation of several environmental estrogens, polybrominated diphenyl ethers (PBDEs), phthalate esters (PAEs) and bisphenol A (BPA), in human serum by gas chromatography-mass spectrometry (GC-MS). After denaturation of protein by concentrated hydrochloride acid, extraction by ether, separation by silica flash chromatograph, several group components including PBDEs, PAEs and BPA in human serum were separated and purified. Then these group components were detected by GC-MS in selected ion monitoring mode. The standard calibration curves of PBDEs, PAEs and BPA were linear over the assayed range with correlation coefficients equal or higher than 0.99. The detection limitations of the targeted compounds ranged from 0.005 to 0.048 mu g L-1 for PBDEs, 0.103-0.833 mu g L-1 for PAEs, and 0.035 mu g L-1 for BPA. In standard duplicated samples, the relative standard deviations were 2.8%-10.9% for PBDEs, 5.6%-9.9% for PAEs, and 3.0% for BPA. The surrogate recoveries in these 7 samples ranged from 74.8% to 88.5% for PCB-209 (polychloride diphenyl ether 209) in PBDEs, from 78.7% to 97.0% for DBP-D4 (dibutyl phthalate-deutorium 4) in PAEs, and from 76.3 % to 93.1% for BPA-D16 (Bisphenol A-Deutorium 16) in BPA, respectively. This method for detecting multiple environmental estrogens in human serum is sensitive, high reproducible with satisfied recoveries.