US EPA

1453461 

Journal Article 

Simultaneously preparative purification of Huperzine A and Huperzine B from Huperzia serrata by macroporous resin and preparative high performance liquid chromatography 

Zhang, H; Liang, Hao; Kuang, P; Yuan, Q; Wang, Yan 

2012 

Yes 

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN: 1570-0232
EISSN: 1873-376X 

904 

65-72 

English 

22877738 

10.1016/j.jchromb.2012.07.019 

WOS:000308734400008 

Huperzine A (HupA) and Huperzine B (HupB) are natural
alkaloids existed in Lycopodium plants. They both have potential clinical application for
treating Alzheimer's Disease (AD). For the purpose of better utilizing the limited plant
resources, a quick and low cost method to separate and purify HupA and HupB from Huperzia serrata
(Thunb. ex Murray) was established in this paper. Low polarity macroporous resin SP850 was
selected from eight kinds of resins during initial purification. Trifluoroacetic acid (TFA) was
proved to be the best acid modifier reagent among all acids used in our experiment for improving
separation. HupA and HupB were baseline separated on a C18 column by preparative high performance
liquid chromatography (Preparative HPLC), the optimal gradient mobile phase system contained
methanol increasing from 15% (v/v) to 35% (v/v) and 0.1% (v/v) TFA within the water. The purity
of HupA and HupB obtained was 99.1% and 98.6%, respectively, and the total recovery for them was
83.0% and 81.8%, respectively. (C) 2012 Elsevier B.V. All rights reserved. 

Huperzine A; Huperzine B; Huperzia serrata; Macroporous resin; Preparative HPLC; Trifluoroacetic acid