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HERO ID
1487686
Reference Type
Journal Article
Title
H-1 NMR metabolomics of earthworm responses to sub-lethal PAH exposure
Author(s)
Brown, SAE; Simpson, AJ; Simpson, MJ
Year
2009
Is Peer Reviewed?
Yes
Journal
Environmental Chemistry
ISSN:
1448-2517
EISSN:
1449-8979
Volume
6
Issue
5
Page Numbers
432-440
Language
English
DOI
10.1071/EN09054
Web of Science Id
WOS:000271005600009
URL
https://www.proquest.com/scholarly-journals/¹h-nmr-metabolomics-earthworm-responses-sub/docview/46421263/se-2?accountid=171501
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Abstract
Metabolic responses of earthworm exposure to the polycyclic
aromatic hydrocarbons (PAHs) naphthalene, phenanthrene and pyrene in contact tests were measured
using H-1 nuclear magnetic resonance (NMR). Novel metabolites were not detected but principal
component analysis (PCA) showed that earthworms exposed to 10, 50 and 100 mu g cm(-2)
naphthalene, phenanthrene and pyrene differed from unexposed (control) earthworms. Partial
least-squares-discriminant analysis (PLS-DA) showed that earthworms had statistically significant
responses to PAH exposure, except for 10 mu g cm(-2) naphthalene and 50 mu g cm(-2) pyrene.
Leucine, valine, alanine, lysine and maltose were identified as potential response indicators of
PAH exposure, but whether the concentration of these metabolites increased or decreased was PAH-
and concentration-dependent. These initial findings reveal the potential of metabolomics for
monitoring earthworm responses to sub-lethal PAH exposure and highlight the role of metabolomics
as a future tool in ecotoxicology.
Keywords
contact tests; Eisenia fetida; metabolic profiling; metabonomics
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Naphthalene
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Naphthalene (2021 Evidence mapping publication)
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