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HERO ID
1495938
Reference Type
Journal Article
Title
In vitro shoot regeneration of fenugreek (Trigonella foenum-graecum L.) using different cytokinins
Author(s)
Aasim, M; Hussain, N; Umer, EM; Zubair, M; Hussain, SB; Saeed, S; Rafique, TS; Sancak, C
Year
2010
Is Peer Reviewed?
Yes
Journal
African Journal of Biotechnology
ISSN:
1684-5315
Volume
9
Issue
42
Page Numbers
7174-7179
Web of Science Id
WOS:000283197400023
Abstract
Fenugreek (Trigonella foenum-graecum L.) is an important
vegetable, spice and medicinal legume plant used as fresh and dried leaves and seeds in many
parts of the world. Fenugreek seeds contain polysaccharide galactomannan and different saponins
and have anti-microbial activity. The study presents an efficient shoot regeneration protocols
from 8 - 10 days old in vitro grown cotyledon node explants cultured on Murashige and Skoog (MS)
medium supplemented with 0.05 - 0.80 mg/l kinetin, 0.25 - 1.0 mg/l 6 benzyladenine (BA) with 0
and 0.20 mg/l alpha naphtalene acetic acid (NAA) and 0.05 - 0.80 mg/l thidiazuran (TDZ) with 0
and 0.10 mg/l indole 3 butyric acid (IBA). All culture mediums were solidified with 0.22%
gelrite. Maximum shoot regeneration and number of shoots per explant were recorded on MS medium
supplemented with TDZ with or without IBA. Maximum of 22.21 shoots per explant were recorded on
MS medium containing 0.40 mg/l TDZ. Presence of auxins in the culture medium positively increased
the mean shoot length. Regenerated shoots were transferred to rooting media containing 0.1 - 1.0
mg/l IBA or NAA.
Keywords
Fenugreek; cotyledon node; hypocotyl; shoot regeneration
Tags
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Naphthalene
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Naphthalene (2021 Evidence mapping publication)
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