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HERO ID
1508687
Reference Type
Journal Article
Title
Characterization of arsenic-induced cytotoxicity in liver with stress in erythrocytes and its reversibility with Pleurotus florida lectin
Author(s)
Rana, T; Bera, AK; Bhattacharya, D; Das, S; Pan, D; Das, SK
Year
2015
Is Peer Reviewed?
1
Journal
Toxicology and Industrial Health
ISSN:
0748-2337
EISSN:
1477-0393
Volume
31
Issue
2
Page Numbers
108-122
Language
English
PMID
23282998
DOI
10.1177/0748233712468026
Web of Science Id
WOS:000348905300002
Abstract
Arsenic is one of the most hazardous substances in the environment known to cause toxicity in multiple organs. Cell adhesion, morphological alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3/CPP32 fluorometric protease assay were important biomarkers to assess apoptosis in cells. This study aimed to evaluate arsenic-induced apoptosis in the hepatocytes of rat and its protective efficacy with coadministration of ascorbic acid (AA) and Pleurotus florida lectin (PFL) individually. Results of the present study also showed that arsenic caused cytotoxicity by elevating morphological alterations, TUNEL-positive nuclei, caspase-3 activity and DNA damage and reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in hepatocytes was reverted to normal value after coadministration of mushroom lectin in arsenic-exposed rat. The study provided significant evidence that PFL has antiapoptotic property against arsenic-induced toxicity. The beneficial effect of PFL was proportional to its duration of exposure. Retard activities of superoxide dismutase and catalase, enhanced lipid peroxidation as well as protein carbonyl in erythrocytes caused by arsenic could also be maintained toward normalcy by supplementation of AA and PFL. These antioxidative effects were exhibited in a time-dependant manner. In rat, treatment with AA and PFL prevented alteration of plasma enzyme activities caused by arsenic. The results concluded that treatment with PFL has significant role in protecting animals from arsenic-induced erythrocytic damage. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.
Keywords
Arsenic; Pleurotus florida lectin; stress; erythrocytes; apoptosis; hepatocytes
Tags
•
Arsenic Hazard ID
PubMed
Considered New
2. Lit Search Updates through Oct 2015
PubMed
Considered
Lit Search Updates Oct 2015 to Jan 2019
ToxNet
WOS
2.5 Update 2015-2019: Title & Abstract Screening
Not relevant to PECO
7. Other Studies through Oct 2015
MOA
Not Relevant
•
Arsenic (Inorganic)
1. Literature
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
4. Adverse Outcome Pathways/Networks Screening
Relevant
•
Arsenic MOA
4. Adverse Outcome Pathways
Cell viability, cell proliferation, cell cycle changes (unrelated to regenerative proliferation)
5. Health Effect
Liver Effects
1. MOA Literature Screening
Health Effect Screening
•
Arsenic Susceptibility
Life Stages Citation Mapping
25%-30%
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