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1510756 
Journal Article 
Efficient cryopreservation protocol enables accessibility of a broad range of ammonia-oxidizing bacteria for the scientific community 
Hoefman, S; Pommerening-Röser, A; Samyn, E; De Vos, P; Heylen, K 
2013 
1 
Research in Microbiology
ISSN: 0923-2508
EISSN: 1769-7123 
164 
4 
288-292 
English 
23376087 
WOS:000319632300002 
Long-term storage of the fastidious ammonia-oxidizing bacteria has proven difficult, which limits their public availability and results in a loss of cultured biodiversity. To enable their accessibility to the scientific community, an effective protocol for cryopreservation of ammonia-oxidizing cultures at -80 °C and in liquid nitrogen was developed. Long-term storage could be achieved using 5% DMSO as cryoprotectant, preferably in a cryoprotective preservation medium containing tenfold-diluted trypticase soy broth and 1% trehalose. As such, successful activity and growth recovery was observed for a diverse set of ammonia-oxidizing cultures. 
Long-term preservation; Ammonia-oxidizing bacteria; Cryoprotective agent