US EPA

1539833 

Journal Article 

Susceptibility to cardiac ischemia/reperfusion injury modulated by an estrogen derivative 

Figueroa-Valverde, L; Diaz-Cedillo, F; Garcia-Cervera, E; Pool-Gomez, E; Lopez-Ramos, M 

2012 

6 

33 

2493-2502 

10.5897/AJPP12.293 

WOS:000310808800009 

Several studies indicate that some steroid derivatives have
activity at cardiovascular level; nevertheless, there is scarce information about the effects of
the estradiol derivatives on cardiac injury ischemia/reperfusion (I/R). Therefore, in this study,
an estradiol derivative was synthetized with the objective of evaluating its activity on I/R in
an ischemia-reperfusion model. In addition, molecular mechanism involved in the activity of
effect induced by estradiol derivative on perfusion pressure and coronary resistance was
evaluated using the Langendorff technique by measuring left ventricular pressure in absence or
presence of the following compounds; tamoxifen, yohimbine, ICI 118,551 and L-NAME. The results
showed that estradiol derivative reduced infarct size compared with control. In addition, another
results showed that the estradiol derivative significantly decrease the perfusion pressure and
coronary resistance in isolated heart. Additionally, another data indicate that estradiol
derivative low left ventricular pressure in a dose-dependent manner (1 x 10(-9) to 1 x 10(-4)
mmol); however, this phenomenon was significantly inhibited by tamoxifen at a dose of 1 x 10(-6)
mmol and L-NAME (1 x 10(-6) mmol). In conclusion, these data suggest that cardioprotective effect
of estradiol derivative is through the interaction with estrogen receptor and activation of
nitric oxide synthase. This phenomenon results in decrease of myocardial necrosis after ischemia
and reperfusion. 

Estradiol derivative; ischemia/reperfusion; tamoxifen; L-NAME