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HERO ID
155123
Reference Type
Journal Article
Title
ROS scavenging effects of organic extract of diesel exhaust particles on human neutrophil granulocytes and rat alveolar macrophages
Author(s)
Aam, BB; Fonnum, F
Year
2007
Is Peer Reviewed?
1
Journal
Toxicology
ISSN:
0300-483X
EISSN:
1879-3185
Volume
230
Issue
2-3
Page Numbers
207-18
Language
English
PMID
17175087
DOI
10.1016/j.tox.2006.11.057
Web of Science Id
WOS:000244376900011
Abstract
Diesel exhaust particles are major constituents of ambient air pollution, and are associated with respiratory and cardiovascular diseases and lung cancer. The organic part of the particles is heterogenic and complex, and seems to be responsible for many of the adverse effects.
Increased formation of ROS is often connected to the adverse effects. We have therefore investigated the effect of an organic extract of diesel exhaust particles on the reactive oxygen species (ROS) status in human neutrophil granulocytes and rat alveolar macrophages in vitro. ROS formation were studied by three different assays namely the use of DCFH-DA, lucigenin and luminol. The organic extract increased ROS assayed with DCFH-DA, but it decreased the amount of ROS in cells stimulated by PMA in all three assays. The identities of the ROS affected were further studied in cell free systems. The cell free studies confirmed that the extract had scavenging effects against superoxide, hypochlorite and to a smaller extent against peroxynitrite, but not against the hydroxyl radical and nitric oxide. ROS take part in the intracellular signalling pathways as well as in the defence against invading microorganisms, and the possible effects of interference of the redox status in the cells are discussed.
Keywords
diesel particulate extracts (DPE); neutrophil granulocytes (NG); alveolar macrophages (AM); reactive oxygen species (ROS); ROS scavenging effect
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