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HERO ID
1598470
Reference Type
Journal Article
Title
GLP-compliant evaluation and standardization of the peripubertal castrate male rat Hershberger assay for oral exposure of test agents
Author(s)
Sloan, CS; Vick, KD; Kuney, NM; Fail, PA; Becker, RA; Tyl, RW
Year
2013
Is Peer Reviewed?
1
Journal
Reproductive Toxicology
ISSN:
0890-6238
EISSN:
1873-1708
Volume
35
Page Numbers
108-116
Language
English
PMID
23123233
DOI
10.1016/j.reprotox.2012.10.006
Web of Science Id
WOS:000314003900014
Abstract
Since oral exposure is more relevant than the sc route for human exposure to environmental substances, studies to evaluate and standardize this route in the Hershberger assay were conducted in 2001-2003. Interest in environmental androgen agonists is increasing, so the oral route of the Hershberger assay may be useful to quantify agonist activity of these substances. Castrated Sprague-Dawley rats were dosed (PND 60-69) with androgen receptor agonists and/or antagonists, terminated on PND 70, and body, liver, and accessory sex organs (ASOs) weighed. Methyltestosterone (MT) po, at 0.1-50 mg/kg/day, resulted in dose-dependent increases in ASO weights at 5-50 mg/kg; 0.1 mg/kg/day was without statistically significant effect. Testosterone propionate (TP) (sc) at 0.1-1.6 mg/kg/day also resulted in dose-dependent increases in ASO weights, at all doses. Detection of putative androgen antagonists by the oral route was confirmed with dose-response curves of antagonism from flutamide (FLU) po at 1, 5, or 10 mg/kg/day, with MT at 5 or 10 mg/kg/day (po, 4 h later). These results extend the OECD Hershberger assay evaluation and standardization to the oral route and identify and discuss challenges of the assay to detect (anti)androgen-active compounds. (C) 2012 Elsevier Inc. All rights reserved.
Keywords
Castrated; Peripubertal; Rat; Hershberger assay; Androgens; Anti-androgens
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Phthalates – Targeted Search for Epidemiological Studies
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Source – no date limit through June 2013 (Private)
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Source - Dec 2013 Update (Private)
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Source - Jun 2014 Update (Private)
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