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HERO ID
1808388
Reference Type
Journal Article
Title
Fragrance and contact allergens in vitro modulate the HLA-DR and E-cadherin expression on human epidermal Langerhans cells
Author(s)
Verrier, A; Schmitt, D; Staquet, M
Year
1999
Is Peer Reviewed?
Yes
Journal
International Archives of Allergy and Immunology
ISSN:
1018-2438
EISSN:
1423-0097
Volume
120
Issue
1
Page Numbers
56-62
Language
English
PMID
10529589
Web of Science Id
WOS:000082990200007
Abstract
BIOSIS COPYRIGHT: BIOL ABS. Background: Epidermal Langerhans cells (LCs) play a critical role in the induction of contact hypersensitivity. The LCs leave the skin, move to the regional lymph nodes and present the allergens embedded in the HLA-DR molecule to naive T-lymphocytes. To allow LC emigration from the epidermis, E-cadherin must be down-regulated. In this study, we have examined the early events that occur in the human epidermis after exposure to three strong contact sensitizers and two commonly used fragrances by e nesulfonic acid, Bandrowski's base, or p-phenylene diamine resulted in a dose-dependent decrease of HLA-DR expression on the surface of LCs without affecting the number of positive cells. These contact allergens induced a downregulation of E-cadherin expression as well as a significant decrease of the percentage of E-cadherin-positive cells. Incubation with an irritant, sodium lauryl sulfate, did not significantly change HLA-DR and E-cadherin expression. Conclusions: Based on the alteration of E s of in vitro tests, this rapid and simple method should help to detect the sensitizing potential of a substance to be applied onto the human skin as an alternative to animal testing.
Keywords
Biochemical Studies-General
;
Integumentary System-General
;
Immunology and Immunochemistry-General
;
Allergy
;
Hominidae
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