Perfluoroalkylated compounds induce cell death and formation of reactive oxygen species in cultured cerebellar granule cells | Health & Environmental Research Online (HERO)

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Citation
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HERO ID

2150117

Reference Type

Journal Article

Title

Perfluoroalkylated compounds induce cell death and formation of reactive oxygen species in cultured cerebellar granule cells

Author(s)

Reistad, T; Fonnum, F; Mariussen, E

Year

2013

Is Peer Reviewed?

Journal

Toxicology Letters
ISSN: 0378-4274
EISSN: 1879-3169

Publisher

ELSEVIER IRELAND LTD

Location

CLARE

Volume

218

Issue

Page Numbers

56-60

Language

English

PMID

23340305

DOI

10.1016/j.toxlet.2013.01.006

Web of Science Id

WOS:000315357400009

URL

https://www.proquest.com/scholarly-journals/perfluoroalkylated-compounds-induce-cell-death/docview/1312842566/se-2?accountid=171501
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Abstract

The present communication investigates the effects of different perfluoroalkylated compounds (PFCs) on formation of reactive oxygen species (ROS) and cell death in cultured cerebellar granule cells. This allows direct comparison with similar effects found for other environmental contaminants like polychlorinated biphenyls and brominated flame-retardants. The increase in ROS formation and cell death was assayed using the fluorescent probe 2,7-dichlorofluorescin diacetate (DCFH-DA) and the trypan blue exclusion assay. The effects of the PFCs were structure dependent. Cell death was induced at relatively low concentrations by perfluorooctyl sulfonate (PFOS), perfluorooctane sulfonylamide (PFOSA) and the fluorotelomer alcohol 1H, 1H, 2H, 2H-perfluorodecanol (FTOH 8:2) with EC(50)-values of 62 ± 7.6, 13 ± 1.8 and 15 ± 4.2 μM (mean ± SD) respectively. PFOS, perfluorooctanoic acid (PFOA) and PFOSA induced a concentration dependent increase in ROS formation with EC(50)-values of 27 ± 9.0, 25 ± 11 and 57 ± 19μM respectively. Reduced cell viability and ROS formation were observed at concentration level close to what is found in serum of occupationally exposed workers. The effect of PFCs on ROS formation and cell viability was compared with other halogenated compounds and future investigations should emphasize effects of mixtures and how physical chemical properties of the compounds influence their toxicity.

Keywords

Perfluoroalkylated compounds (PFC); PFOS; FTOH; PFOA; PFOSA; Cerebellar granule cells; Reactive oxygen species (ROS); Cell death

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