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Citation
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HERO ID
2150117
Reference Type
Journal Article
Title
Perfluoroalkylated compounds induce cell death and formation of reactive oxygen species in cultured cerebellar granule cells
Author(s)
Reistad, T; Fonnum, F; Mariussen, E
Year
2013
Is Peer Reviewed?
1
Journal
Toxicology Letters
ISSN:
0378-4274
EISSN:
1879-3169
Publisher
ELSEVIER IRELAND LTD
Location
CLARE
Volume
218
Issue
1
Page Numbers
56-60
Language
English
PMID
23340305
DOI
10.1016/j.toxlet.2013.01.006
Web of Science Id
WOS:000315357400009
URL
https://www.proquest.com/scholarly-journals/perfluoroalkylated-compounds-induce-cell-death/docview/1312842566/se-2?accountid=171501
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Abstract
The present communication investigates the effects of different perfluoroalkylated compounds (PFCs) on formation of reactive oxygen species (ROS) and cell death in cultured cerebellar granule cells. This allows direct comparison with similar effects found for other environmental contaminants like polychlorinated biphenyls and brominated flame-retardants. The increase in ROS formation and cell death was assayed using the fluorescent probe 2,7-dichlorofluorescin diacetate (DCFH-DA) and the trypan blue exclusion assay. The effects of the PFCs were structure dependent. Cell death was induced at relatively low concentrations by perfluorooctyl sulfonate (PFOS), perfluorooctane sulfonylamide (PFOSA) and the fluorotelomer alcohol 1H, 1H, 2H, 2H-perfluorodecanol (FTOH 8:2) with EC(50)-values of 62 ± 7.6, 13 ± 1.8 and 15 ± 4.2 μM (mean ± SD) respectively. PFOS, perfluorooctanoic acid (PFOA) and PFOSA induced a concentration dependent increase in ROS formation with EC(50)-values of 27 ± 9.0, 25 ± 11 and 57 ± 19μM respectively. Reduced cell viability and ROS formation were observed at concentration level close to what is found in serum of occupationally exposed workers. The effect of PFCs on ROS formation and cell viability was compared with other halogenated compounds and future investigations should emphasize effects of mixtures and how physical chemical properties of the compounds influence their toxicity.
Keywords
Perfluoroalkylated compounds (PFC); PFOS; FTOH; PFOA; PFOSA; Cerebellar granule cells; Reactive oxygen species (ROS); Cell death
Tags
IRIS
•
PCBs
Litsearches
Remaining
LitSearch August 2015
Pubmed
Toxline
WoS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
PubMed
Web of Science
Screened Studies
Excluded
Exclude (TIAB)
Perfluorooctane
Potassium perfluorooctanoate
Sodium perfluorooctanoate
•
FtOH 8:2
Literature Search
Pubmed
WOS
Screening Results
In vitro/ex vivo/in silico
Neurotox mech
•
^Per- and Polyfluoroalkyl Substances (PFAS)
PFOSA (754-91-6)
Literature Search
Pubmed
WOS
FtOH 8:2 (678-39-7)
Literature Search
Pubmed
WOS
•
PFAS 150
Literature Search Update December 2020
PubMed
WOS
Literature Search August 2019
PubMed
Web of Science
Screened Studies
Supplemental
8:2 Fluorotelomer alcohol
Ammonium perfluorooctanoate
Perfluorooctane
Perfluorooctanesulfonamide
Perfluorooctanesulfonamide sodium salt (1:1)
Perfluorooctanesulfonate
Perfluorooctanesulfonic acid
Perfluorooctanoic acid
•
PFOA (335-67-1) and PFOS (1763-23-1)
Literature Search Update (2013-2019)
PubMed
WOS
•
PFOSA
Literature Search
Pubmed
WOS
Screening Results
In vitro/ex vivo/in silico
Neurotox mech
Other mechanistic studies
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