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2213542 
Technical Report 
CHARACTERIZATION OF THE IN VITRO UNSCHEDULED DNA SYNTHESIS ASSAY IN PRIMARY LUNG CELLS OF THE RAT 
Whong, WZ; Stewart, JD; Ong, T 
1991 
1 
Mutation Research Letters
ISSN: 0165-7992 
EMICBACK/77903 
51 
1 
51-55 
English 
The in vitro unscheduled DNA synthesis (UDS) assay has been evaluated in rat primary lung cells with known genotoxicants. The autoradiographic method was employed to detect UDS in both alveolar macrophages and primary pulmonary cells. Data of a time course study revealed that a high radioactive labeling of DNA repair was achieved after a 16-h incubation with [3H]thymidine. Coupled with low serum (1%), hydroxyurea at the concentration of 20 mM inhibited regular DNA synthesis in primary lung cells in a satisfactory manner (81-88% inhibition). With this protocol, a dose-related increase in UDS was induced by N-methyl-N′-nitro-N-nitrosoguanidine and 2-aminoanthracene in both rat alveolar macrophages and primary lung cells. The results suggest that primary rat lung cells in culture possess DNA-repair ability and that the UDS assay may be useful for assessing the pulmonary genotoxic effect of chemicals in this cell system. © 1991. 
(Rat); Alveolar macrophages; DNA repair; Primary lung cells; Unscheduled DNA synthesis (UDS) 
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