Biodegradation of fuel oxygenates and their effect on the expression of a newly identified cytochrome P450 gene in Achromobacter xylosoxidans MCM2/2/1 | Health & Environmental Research Online (HERO)

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HERO ID

2321114

Reference Type

Journal Article

Title

Biodegradation of fuel oxygenates and their effect on the expression of a newly identified cytochrome P450 gene in Achromobacter xylosoxidans MCM2/2/1

Author(s)

Gunasekaran, V; Donmez, E; Girhard, M; Urlacher, VB; Constanti, M

Year

2014

Is Peer Reviewed?

Journal

Process Biochemistry
ISSN: 1359-5113
EISSN: 0032-9592

Volume

Issue

Page Numbers

124-129

DOI

10.1016/j.procbio.2013.09.028

Web of Science Id

WOS:000331156100018

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84891826426&doi=10.1016%2fj.procbio.2013.09.028&partnerID=40&md5=e44a81da901be4a9a10924494608115c
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Abstract

Achromobacter xylosoxidans MCM2/2/1 was enriched and isolated from gasoline-contaminated soil and was found to degrade ethyl tert-butyl ether (ETBE) and methyl tert-butyl ether (MTBE) by 41.48% and 34.15%, respectively, in 6 days. Furthermore, the effect of MTBE and TBA on the expression of cytochrome P450 (CYP) of A. xylosoxidans MCM2/2/1 was examined. The presence of the CYP gene in this organism was first confirmed by amplification of a putative 350 bp CYP gene fragment followed by identification of the entire gene by genome walking and DNA-sequencing. The identified CYP gene of A. xylosoxidans MCM2/2/1 shares a high similarity of about 88% with the thcB gene of A. xylosoxidans A8. Gene expression studies have shown that the CYP gene is expressed in A. xylosoxidans MCM2/2/1; however, the expression of this gene was altered at different concentrations of MTBE. © 2013 Elsevier Ltd.

Keywords

Achromobacter xylosoxidans; MTBE; ETBE; Biodegradation; Cytochrome P450