US EPA

2509297 

Book/Book Chapter 

Effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP 

Li, H; Zhang, Yi; Wang, P; Yu, J 

2014 

Yes 

Advanced Materials Research
ISSN: 1022-6680 

Trans Tech Publications 

Zurich, Switzerland 

Environmental engineering : selected, peer reviewed papers from the 3rd International Conference on Energy, Environment and Sustainable Development (EESD 2013), November 12-13, 2013, Shanghai, China 

864-867 

797-800 

English 

10.4028/www.scientific.net/AMR.864-867.797 

WOS:000339362700162 

Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic effects, is widely used in many industries such as plastics, pharmaceuticals, plastic food packaging materials. This article intends to study the effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP via vitro test. Testicular sertoli cells of rates were incubated by medium containing DBP in different concentrations (0 mu g/ml,1 mu g/ml,10 mu g/ml, 100 mu g/ml). after culturing for 24 hours the expression of vimentin was detected by immunofluorescence; the expression of vimentin mRNA detected by RT-PCR; Western blot analyzed the expression of related protein of JNK/MAPK pathways in sertoli cells. The results showed that vimentin highly express in sertoli cells and gradually decrease with the increase of poisonous doses. The mRNA expression of vimentin in each exposure group has a downtrend with the increase of does. The total protein of INK and P-INK protein increased gradually in a dose effect manner. These results suggested that DBP can decrease the expression of vimentin mRNA, the decrease of which was regulated by JNK/MAPK signal transduction pathway that activated by DBP, and then induced the apoptosis of Sertoli cell. 

DBP; testicular sertoli cells; JNK/MAPK; vimentin 

Li, H; Xu, Q; Ge, H 

Advanced materials research, v. 864-867 

9783037859735 

3rd International Conference on Energy, Environment and Sustainable Development (EESD 2013) 

Shanghai, China 

November 12-13, 2013