US EPA

2713226 

Journal Article 

Improved micropropagation protocol for leatherleaf fern (Rumohra adiantiformis) using rhizomes as donor explant 

Winarto, B; da Silva, JAT 

2012 

1 

Scientia Horticulturae
ISSN: 0304-4238
EISSN: 1879-1018 

140 

74-80 

English 

10.1016/j.scienta.2012.03.017 

WOS:000305312600012 

http://://WOS:000305312600012
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A new micropropagation protocol for leatherleaf fern (Rumohra adiantiformis (G. Forst.) Ching) was successfully established using rhizomes as the donor explant, following appropriate sterilization. Murashige and Skoog (MS) medium containing 0.25 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), 0.2 mg/l alpha-naphthalene acetic acid (NAA), 1.0 mg/16-benzyl adenine (BA), and 0.5 mg/l thidiazuron (TDZ) with 30 g/l sucrose (IM-4) was the most appropriate medium for culture initiation. When entire rhizomes harvested from mother stock plants were cultured on a simple paper bridge containing liquid IM-4 medium, culture initiation improved with 3.4 rhizomes regenerated per sub-culture. The average multiplication rate of newly regenerated rhizomes increased to 5.7/rhizome on half-strength MS medium supplemented with 0.05 mg/l IAA, 0.25 mg/l BA, 0.5 mg/l Kin, 1 g/l activated charcoal and 20 g/l sucrose (MM-5). The level of multiplication peaked in the fifth subculture and retained high quality until the sixth subculture. From the seventh subculture onwards, the quality of regenerated fronds was reduced. The regenerated rhizomes rooted easily on MM-5 and could be acclimatized ex vitro with 97-100% survival. (C) 2012 Elsevier B.V. All rights reserved. 

Rhizome; Frond development; Simple paper bridge; Multiplication; Murashige and Skoog medium; Leatherleaf fern (Rumohra adiantiformis)