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2835811 
Journal Article 
Polyethylene glycol rinse solution: an effective way to prevent ischemia-reperfusion injury 
Zaouali, MA; Bejaoui, M; Calvo, M; Folch-Puy, E; Pantazi, E; Pasut, G; Rimola, A; Ben Abdennebi, H; Adam, R; Roselló-Catafau, J 
2014 
1 
World Journal of Gastroenterology
ISSN: 1007-9327
EISSN: 2219-2840 
20 
43 
16203-16214 
English 
25473175 
WOS:000346050500028 
AIM: To test whether a new rinse solution containing polyethylene glycol 35 (PEG-35) could prevent ischemia-reperfusion injury (IRI) in liver grafts.

METHODS: Sprague-Dawley rat livers were stored in University of Wisconsin preservation solution and then washed with different rinse solutions (Ringer's lactate solution and a new rinse solution enriched with PEG-35 at either 1 or 5 g/L) before ex vivo perfusion with Krebs-Heinseleit buffer solution. We assessed the following: liver injury (transaminase levels), mitochondrial damage (glutamate dehydrogenase activity), liver function (bile output and vascular resistance), oxidative stress (malondialdehyde), nitric oxide, liver autophagy (Beclin-1 and LCB3) and cytoskeleton integrity (filament and globular actin fraction); as well as levels of metalloproteinases (MMP2 and MMP9), adenosine monophosphate-activated protein kinase (AMPK), heat shock protein 70 (HSP70) and heme oxygenase 1 (HO-1).

RESULTS: When we used the PEG-35 rinse solution, reduced hepatic injury and improved liver function were noted after reperfusion. The PEG-35 rinse solution prevented oxidative stress, mitochondrial damage, and liver autophagy. Further, it increased the expression of cytoprotective heat shock proteins such as HO-1 and HSP70, activated AMPK, and contributed to the restoration of cytoskeleton integrity after IRI.

CONCLUSION: Using the rinse solution containing PEG-35 was effective for decreasing liver graft vulnerability to IRI. 
Liver washout; Liver transplantation; Rinse solution; Ischemia-reperfusion injury; Polyethylene glycol 35; Nitric oxide; Adenosine monophosphate-activated protein kinase; Heme oxygenase 1; Heat shock; protein 70; Metalloproteinases