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2849802 
Journal Article 
Three phase partitioning for simultaneous purification of aloe polysaccharide and protein using a single-step extraction 
Tan, Z; Wang, C; Yi, YJ; Wang, HY; Zhou, W; Tan, S; Li, F 
2015 
1 
Process Biochemistry
ISSN: 1359-5113
EISSN: 0032-9592 
Elsevier B.V., The Boulevard Kidlington Oxford OX5 1GB United Kingdom 
50 
3 
482-486 
English 
WOS:000352745300019 
In this paper, an inexpensive, rapid and efficient three phase partitioning (TPP) technique was used to simultaneously purify aloe polysaccharide (APS) and protein using a single-step extraction. This TPP system is established by adding ammonium sulfate and t-butanol to the crude slurry of aloe powder. APS was extracted in the lower phase, and the protein was extracted in the middle phase. The extraction conditions optimized were mass concentration of ammonium sulfate and t-butanol, temperature and pH. APS was further purified using a dialysis membrane to remove the salt, and its purity was determined. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze aloe protein. TPP is an attractive and potential technique for the purification of APS and protein from Aloe vera L. (C) 2015 Elsevier Ltd. All rights reserved. 
Three phase partitioning; Aloe polysaccharide; Aloe protein; Purification 
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• tert-Butanol
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