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HERO ID
2849963
Reference Type
Journal Article
Title
Physiological and genetic screening methods for the isolation of methyl tert-butyl ether-degrading bacteria for bioremediation purposes
Author(s)
Guisado, IM; Purswani, J; Gonzalez-Lopez, J; Pozo, C
Year
2015
Is Peer Reviewed?
1
Journal
International Biodeterioration & Biodegradation
ISSN:
0964-8305
EISSN:
1879-0208
Volume
97
Page Numbers
67-74
DOI
10.1016/j.ibiod.2014.11.008
Web of Science Id
WOS:000348557700009
Abstract
Bioremediation of groundwater contaminated with methyl tert-butyl ether (MTBE) has been widely described since their cost/efficient ratios are lower than other physic-chemical methodologies. The present study focused on the isolation and selection of MTBE degrading microorganisms from contaminated soil and groundwater samples based on results from growth on mineral media amended with MTBE and BTEX, presence or absence of the monooxygenase genes and specific ability to degrade MTBE. Three bacterial strains were selected and identified as Rhodococcus ruber, strains EE1 (CECT 8555), EE6 (CECT 8612) and A5 (CECT 8556), showing the ability to degrade 60.0, 36.0 and '10.0 mg l(-1) MTBE, respectively. Moreover, all the R. ruber strains showed the presence of genes encoding MTBE-degrading enzymes. One isolated strain was identified as Paenibacillus sp. SH7 (CECT 8558) and demonstrated the greatest MTBE degradation value (100 mg l(-1)), but together with the last strain selected and identified as Agrobacterium sp. MS2 (CECT 8557) did not result in positive amplification of any of the monooxygenase primers tested. The lowest toxicity (as EC50) was observed after 4-days growth of R. ruber EE6 on MTBE-supplemented mineral medium. The potential application of these strains in bioremediation processes is discussed. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords
MTBE; Bioremediation; Monooxygenases; Ecotoxicity; BTEX
Tags
IRIS
•
ETBE
Combined Dataset (After duplicates removed electronically)
Excluded / Not on Topic
Other chemical/non ETBE studies
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