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Citation
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HERO ID
2915550
Reference Type
Journal Article
Title
Toxicity study of dibutyl phthalate of Rubia cordifolia fruits: in vivo and in silico analysis
Author(s)
Anantharaman, A; Priya, RR; Hemachandran, H; Akella, S; Rajasekaran, C; Ganesh, J; Fulzele, DP; Siva, R
Year
2016
Is Peer Reviewed?
Yes
Journal
Environmental Toxicology
ISSN:
1520-4081
EISSN:
1522-7278
Volume
31
Issue
9
Page Numbers
1059-1067
Language
English
PMID
25926096
DOI
10.1002/tox.22115
Web of Science Id
WOS:000382933800003
URL
https://search.proquest.com/docview/2000115096?accountid=171501
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Abstract
Natural toxins from plant sources with wide ranges of biological activities reflect the upswing of drug design in the pharmaceutical industry. Rubia cordifolia L. is one of the most important red dye yielding plants. Most of the former researches have focused on the bioactive compounds from the roots of R. cordifolia, while no attention was paid towards the fruits. For the first time, here we report the presence of dibutyl phthalate in the fruits of R. cordifolia. Structural characterization was carried out using Ultraviolet-Visible spectrophotometer (UV-Vis), Fourier transform infrared (FTIR), gas chromatography-mass spectrophotometer (GC-MS), Nuclear magnetic resonance (NMR). Acute toxicity of the crude ethanolic extracts of the R. cordifolia fruits was examined in Swiss albino mice. No mortality was observed in all treated mice with 100, 500, 1000 mg/kg body weight of crude extract of R. cordifolia fruit and it indicates that the LD50 value is higher than 1000 mg/kg body weight. This study exhibited a significant change in the body weight. Alanine transaminase (ALT), total protein, triglycerides, glucose, and also the histopathological analysis of liver for all treated mice showed difference from the control group. The dibutyl phthalate was further evaluated for the toxicity study through in silico analysis. Together, the results highlighted that the toxic potential of R. cordifolia fruits extracts and also the toxicity profile of the fruit should be essential for the future studies dealing with the long term effect in animals. © 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.
Keywords
Alanine Transaminase/metabolism; Animals; Body Weight/drug effects; Dibutyl Phthalate/chemistry/isolation & purification/toxicity; Fruit/chemistry/metabolism; Gas Chromatography-Mass Spectrometry; Liver/drug effects/metabolism/pathology; Magnetic Resonance Spectroscopy; Plant Extracts/chemistry; Plant Roots/chemistry/metabolism; Rubia/chemistry/metabolism; Spectroscopy, Fourier Transform Infrared; Toxicity Tests, Acute; Triglycerides/metabolism; Rubia cordifolia; acute oral toxicity; dibutyl phthalate; fruits extracts; in silico; 2286E5R2KE
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch March 2015 - June 2015
Pubmed
LitSearch Jul 2016 - Jan 2017
Prior search overlap
PubMed
WoS
Excluded: No Primary Data on Health Effects
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