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Citation
Tags
HERO ID
3035745
Reference Type
Journal Article
Title
Alterations in the proteome of the respiratory tract in response to single and multiple exposures to naphthalene
Author(s)
Kültz, D; Li, J; Sacchi, R; Morin, D; Buckpitt, A; Van Winkle, L
Year
2015
Is Peer Reviewed?
1
Journal
Proteomics
ISSN:
1615-9853
EISSN:
1615-9861
Volume
15
Issue
15
Page Numbers
2655-2668
Language
English
PMID
25825134
DOI
10.1002/pmic.201400445
Web of Science Id
WOS:000359061800013
URL
http://
://WOS:000359061800013
Exit
Abstract
Protein adduction is considered to be critical to the loss of cellular homeostasis associated with environmental chemicals undergoing metabolic activation. Despite considerable effort, our understanding of the key proteins mediating the pathologic consequences from protein modification by electrophiles is incomplete. This work focused on naphthalene (NA) induced acute injury of respiratory epithelial cells and tolerance which arises after multiple toxicant doses to define the initial cellular proteomic response and later protective actions related to tolerance. Airways and nasal olfactory epithelium from mice exposed to 15 ppm NA either for 4 h (acute) or for 4 h/day × 7 days (tolerant) were used for label-free protein quantitation by LC/MS/MS. Cytochrome P450 2F2 and secretoglobin 1A1 are decreased dramatically in airways of mice exposed for 4 h, a finding consistent with the fact that CYPs are localized primarily in Clara cells. A number of heat shock proteins and protein disulfide isomerases, which had previously been identified as adduct targets for reactive metabolites from several lung toxicants, were upregulated in airways but not olfactory epithelium of tolerant mice. Protein targets that are upregulated in tolerance may be key players in the pathophysiology associated with reactive metabolite protein adduction. All MS data have been deposited in the ProteomeXchange with identifier PXD000846 (http://proteomecentral.proteomexchange.org/dataset/PXD000846).
Keywords
Animal proteomics; Clara cells; Metabolic activation; Naphthalene; Protein adduct
Tags
IRIS
•
Naphthalene
Database Searches - Nov 2015 (private)
New Nov 2015 (private)
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PubMed
WOS
Combined data set
Data set for title/abstract screening
Excluded - PECO criteria not met (TIAB)
Supplemental material
Mechanistic
Mechanisms of cancer
Acute toxicity studies
Other
•
Mouse Lung Tumor Workshop 2014
Mouse Lung Tumor Initial Search Sept-Oct 2023
PubMed
WoS
•
Naphthalene (2021 Evidence mapping publication)
Database Searches - Nov 2015 (private)
New Nov 2015 (private)
Database Searches
PubMed
WOS
Combined data set
Data set for title/abstract screening
Excluded – PECO criteria not met
Supplemental material
Mechanistic
Mechanisms of cancer
Acute toxicity studies
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