Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3044657

Reference Type

Journal Article

Title

Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles

Author(s)

Guenther, DC; Karmakar, S; Hrdlicka, PJ

Year

2015

Is Peer Reviewed?

Yes

Journal

Chemical Communications
ISSN: 1359-7345
EISSN: 1364-548X

Volume

Issue

Page Numbers

15051-15054

Language

English

PMID

26314212

DOI

10.1039/c5cc06264k

Abstract

Double-stranded oligonucleotides with +1 interstrand zipper arrangements of intercalator-functionalized nucleotides are energetically activated for recognition of mixed-sequence double-stranded DNA. Incorporation of nonyl (C9) bulges at specific positions of these probes, results in more highly affine (>5-fold), faster (>4-fold) and more persistent dsDNA recognition relative to conventional Invader probes.