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3045466 
Journal Article 
An ultra-sensitive monoclonal antibody-based enzyme-linked immunosobent assay for dibutyl phthalate in human urinary 
Zhou, L; Lei, Y; Zhang, D; Ahmed, S; Chen, S 
2016 
Yes 
Science of the Total Environment
ISSN: 0048-9697
EISSN: 1879-1026 
Elsevier 
Sci Total Environ. 2016, Jan 15; 541:570-578. [The Science of the total environment] 
541 
Elsevier 
570-578 
English 
Dibutyl phthalate (DBP) has been extensively used as a plasticizer in many daily products, which is highly toxic to human, notably affecting the reproductive and developmental function. As the previous method is expensive, time-consuming, low sensitivity and just focused on the environment. Present study was aimed to establish an ultra-sensitive and simple method based on good quality monoclonal antibody, applying to evaluate excretion level of DBP in urine samples of Chinese population directly. A monoclonal antibody was generated and characterized after fusion of myeloma cells with spleen cells isolated from BALB/c mouse. The mouse was previously immunized using a specially designed amino derivative of DBP conjugated with bovine serum albumin (BSA) as immunogen. Cross-reactivity values of the monoclonal antibody against DBP, di-isobutyl phthalate (DIBP) were observed 100% and 1.25%, while for dimethyl phthalate (DMP), butyl benzyl phthalate (BBP) and didecyl phthalate (DDP) the values were <0.06%. The standard curve was constructed at 0-50ngmL(-1) and good linearity (R(2)=0.994) was achieved. The observed IC50 (7.34ngmL(-1)) and LOD (0.06ngmL(-1)) values was improved 1000-fold to polyclonal antibody and 5-fold to other monoclonal antibodies. A total 1246 urine samples were analyzed and the detection frequency of DBP was observed 72.87% by ic-ELISA. The 95th percentile and mean concentration of DBP were 12.07 and 3.00ngmL(-1). Acceptable recovery rates of DBP were 97.8-114.3% and coefficients variation 5.93-11.09%. The concentrations of DBP in females were found significantly higher (p<0.05) than males. Similarly, the DBP in middle aged and low educated individuals was found higher (p<0.001) than the others. Considering the adverse health effects, DBP internal exposure in the Chinese population should be reduced. The ic-ELISA method has been proved as a cost effective, specific, and highly sensitive screening tool to detect DBP in urinary samples. 
Dibutyl phthalate; Monoclonal antibody; Urinary samples; Internal exposure 
IRIS
• BBP (Butyl benzyl phthalate)
     Literature Search
          Litsearch Jun 2015-Jan 2016
               Pubmed
               WOS
          Literature Search: January 2016 - June 2016
               Pubmed
               WOS
          Literature Search: July 2016 - January 2017
               WOS
     Excluded: No Primary Data on Health Effects
          Not chemical specific
          Measurement methods
• Dibutyl Phthalate (DBP)
     Database Searches
          Litsearch Jan 2016 - July 2016
               Pubmed
               WOS
          LitSearch Jul 2016 - Jan 2017
               Prior search overlap
               PubMed
               WoS
     Excluded: No Primary Data on Health Effects
          Measurement methods
     Litsearch June 2015 - Jan 2016
          Pubmed
          Web of Science
• Diisobutyl Phthalate (DIBP) Final
     Database Searches
          December 2015 Update
               New for This Search
               Pubmed
          June 2016 Update
               Pubmed
               Toxline
          January 2017 Update
               Web of Science
          July 2017 Update
     No Primary Data on Toxic Effects
          Measurement methods
          Exposure levels
• Phthalates – Targeted Search for Epidemiological Studies
     Source – all searches
          Pubmed
          WOS
     Excluded
     Source – Dec 2015 Update (Private)
          Pubmed
     Source - Jun 2016 Update (Private)
          WOS
     Source - Dec 2016 Update (Private)
          Pubmed
          WOS