Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
3056866
Reference Type
Journal Article
Title
Isolation and Identification of an Antimutagenic Phthalate Derivative Compound from Octopus (Paraoctopus limaculatus)
Author(s)
Cruz-Ramirez, SG; Lopez-Saiz, CM; Plascencia-Jatomea, M; Machi-Lara, L; Rocha-Alonzo, F; Marquez-Rios, E; Burgos-Hernandez, A
Year
2015
Is Peer Reviewed?
1
Journal
Tropical Journal of Pharmaceutical Research
ISSN:
1596-5996
Volume
14
Issue
7
Page Numbers
1257-1264
DOI
10.4314/tjpr.v14i7.19
Web of Science Id
WOS:000359700900019
Abstract
Purpose: To isolate and evaluate the antimutagenic properties of compounds previously identified in octopus (Paraoctopus limaculatus).
Methods: Octopus fractions, previously obtained by a sequential thin layer chromatography (TLC) procedure, were subjected to further fractionation by TLC and their anti-mutagenic activity monitored using Salmonella tester strains TA98 and TA100 with metabolic activation (S9) in Ames test. The isolated fractions were subjected to structural studies by Fourier transformed infrared spectroscopy (FTIR), nuclear magnetic resonance (H-1 and C-13 NMR), and gas chromatography-mass spectrometry.
Results: Five new fractions were obtained from a previously isolated and reported anti-mutagenic octopus fraction. Fractions RB21321b2 and RB21321b3 inhibited > 80 % of the mutagenicity induced by 500 ng AFB1 on both tester strains and were selected for chemical/structural characterization. Data from IR and H-1 and C-13 NMR suggested the presence of phthalate type of compounds. GC-MS analysis revealed 278 m/z for both fractions which is consistent with a butyl isobutyl phthalate structure.
Conclusion: Based on the findings, the compound responsible for the high anti-mutagenic activity of the isolated fraction from octopus is 1-butyl-2-isobutyl-phthalate.
Keywords
Octopus; Anti-mutagenic; Paraoctopus limaculatus; 1-Butyl-2-isobutyl-phthalate
Tags
IRIS
•
Diisobutyl Phthalate (DIBP) Final
Database Searches
December 2015 Update
New for This Search
Web of Science
June 2016 Update
Web of Science
January 2017 Update
No Primary Data on Toxic Effects
Miscellaneous
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity