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Citation
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HERO ID
3070970
Reference Type
Journal Article
Title
The use of titanium dioxide for selective enrichment of phosphorylated peptides
Author(s)
Thingholm, TE; Larsen, MR
Year
2016
Is Peer Reviewed?
1
Journal
Methods in Molecular Biology
ISSN:
1064-3745
EISSN:
1940-6029
Volume
1355
Page Numbers
135-146
Language
English
PMID
26584923
DOI
10.1007/978-1-4939-3049-4_9
Web of Science Id
WOS:000368703600010
Abstract
Titanium dioxide (TiO2) has very high affinity for phosphopeptides and in recent years it has become one of the most popular methods for phosphopeptide enrichment from complex biological samples. Peptide loading onto TiO2 resin in a highly acidic environment in the presence of 2,5-dihydroxybenzoic acid (DHB), phthalic acid, lactic acid, or glycolic acid has been shown to improve selectivity significantly by reducing unspecific binding of non-phosphorylated peptides. The phosphopeptides bound to the TiO2 are subsequently eluted from the chromatographic material using an alkaline buffer. TiO2 chromatography is extremely tolerant towards most buffers used in biological experiments, highly robust and as such it has become the method of choice in large-scale phosphoproteomics. Here we describe a batch mode protocol for phosphopeptide enrichment using TiO2 chromatographic material followed by desalting and concentration of the sample by reversed phase micro-columns prior to downstream MS and LC-MS/MS analysis.
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Not chemical specific
Use in sample prep or assay
Litsearch June 2015 - Jan 2016
Pubmed
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
Excluded
Source - Dec 2016 Update (Private)
Pubmed
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