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HERO ID
3123421
Reference Type
Journal Article
Title
Novel pyrene containing monomeric and dimeric supramolecular AIEE active nano-probes utilized in selective "off-on" trivalent metal and highly acidic pH sensing with live cell applications
Author(s)
Shellaiah, M; Simon, T; Srinivasadesikan, V; Lin, CM; Sun, K; Ko, FuH; Lin, MC; Lin, HC
Year
2016
Journal
Journal of Materials Chemistry C
ISSN:
2050-7526
EISSN:
2050-7534
Publisher
Royal Society of Chemistry
Location
CAMBRIDGE
Volume
4
Issue
10
Page Numbers
2056-2071
Language
English
DOI
10.1039/c5tc03738g
Web of Science Id
WOS:000371671400017
Abstract
Two novel pyrene containing monomeric and dimeric Schiff base derivatives PCS1 and PCS2 have been synthesized via one-pot reaction and their nano-J-type aggregation induced emission enhancement (AIEE) was well demonstrated using UV-Vis/PL, transmission electron microscopy (TEM), dynamic light scattering (DLS), time resolved photoluminescence (TRPL), and live cell imaging studies. In contrast to PCS2, PCS1 in CH3CN exhibits fluorescence "off-on" sensor selectivity towards transition trivalent metal ions (Fe3+, Cr3+ and Al3+) among other metals, via PET inhibition with excimer PCS1-PCS1â formation. The 2 : 1 stoichiometry of PCS1â¯M3+ (M = Fe/Cr/Al) sensor complexes was calculated from Job's plots based on their PL titrations. In addition, the binding sites of PCS1â¯M3+ sensor complexes were well recognised from the 1H NMR titrations and supported by ESI(+ve) mass and FTIR analysis. Additionally, fluorescence reversibilities of PCS1â¯M3+ were observed via consequent addition of M3+ ions and PMDTA, respectively. Furthermore, the detection limits (LODs) and the association constant (Ka) values of PCS1â¯M3+ complexes were calculated using standard deviation and linear fittings. Likewise, quantum yield (Pdbl) measurements, TEM analysis, determination of the effect of pH, density functional theory (DFT) and time resolved photoluminescence (TRPL) studies were performed for the PCS1â¯M3+ sensor complexes. More importantly, confocal fluorescence microscopy imaging of Raw264.7 cells showed that PCS1 could be used as an effective fluorescent probe for detecting transition trivalent metal ions (Fe3+, Cr3+, and Al3+) in living cells. Impressively, both PCS1 and PCS2 showed "off-on" sensing at highly acidic pH values (1-3) with live cell applications. © The Royal Society of Chemistry 2016.
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IRIS
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Ammonia, Oral - Problem Formulation
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