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HERO ID
3123805
Reference Type
Journal Article
Title
Switching monomer/excimer ratiometric fluorescence to time-resolved excimer probe for DNA detection: A simple strategy to enhance the sensitivity
Author(s)
Zhu, Z; Li, W; Yang, C
Year
2016
Is Peer Reviewed?
1
Journal
Sensors and Actuators B: Chemical
ISSN:
0925-4005
EISSN:
1873-3077
Publisher
Elsevier
Location
LAUSANNE
Volume
224
Page Numbers
31-36
Language
English
DOI
10.1016/j.snb.2015.10.004
Web of Science Id
WOS:000366459500004
Abstract
Pyrene has been widely used in designing ratiometric fluorescent probe due to its excimer emission that is different with the monomer in emission wavelengths. To take the advantage of the long-lived excimer emission, a pyrene-based zinc complex is designed as model compound for time-resolved DNA detection. PyZn exhibits excimer emission upon the binding of DNA, and the lifetime of excimer (Ï > 40 ns) is one magnitude longer than that of the monomer. With a delay time of 30 ns, the monomer emission can be eliminated almost completely, leading to a much weaker background and an improved signal-to-noise ratio. Significantly, this excimer-based long-lived fluorescence is not quenched by oxygen, which provides a general strategy to design long-lived fluorescent probes for time-resolved detection in oxygenic environments and organisms. © 2015 Elsevier B.V.
Keywords
Pyrene; Excimer; Time-resolved detection; Fluorescent probes; DNA
Tags
IRIS
•
Ammonia, Oral - Problem Formulation
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