US EPA

3233204 

Journal Article 

Anti-inflammatory effect of propolis through inhibition of nitric oxide production on carrageenin-induced mouse paw edema 

Tan-No, K; Nakajima, T; Shoji, T; Nakagawasai, O; Niijima, F; Ishikawa, M; Endo, Y; Sato, T; Satoh, S; Tadano, T 

2006 

Yes 

Biological and Pharmaceutical Bulletin
ISSN: 0918-6158
EISSN: 1347-5215 

29 

1 

96-99 

WOS:000234760200020 

The anti-inflammatory effect of propolis was compared with
that of diclofenac, a non-steroidal anti-inflammatory drug, and L-N-G-nitro arginine methyl ester
(L-NAME), a nitric oxide synthase inhibitor, using carrageenin-induced mouse paw edema. When
administered 10 min prior to carrageenin injection, propolis (1 : 1000, 1 : 100, p.o.),
diclofenae (12.5, 50 mg/kg, p.o.) and L-NAME (10, 100 mg/kg, s.c.) showed a significant anti-
inflammatory effect. The anti-inflammatory effects of propolis and L-NAME were significantly
inhibited by L-arginine, a precursor of nitric oxide, but not by D-arginine. In contrast, the
anti-inflammatory effect produced by diclofenac was not inhibited by either D-arginine or L-
arginine. These results indicate that the anti-inflammatory effect of propolis on mouse paw edema
acts via the inhibition of nitric oxide production, similar to that of L-NAME but not
diclofenac. 

propolis; carrageenin; paw edema; L-N-G-nitro arginine methyl ester; diclofenac; mouse