US EPA

3350226 

Journal Article 

Separation and purification and in vitro anti-proliferative activity of leukemia cell K562 of Galium aparine L. petroleum ether phase 

Shi, G; Liu, J; Zhao, W; Liu, Y; Tian, X 

2016 

1 

Saudi Pharmaceutical Journal
ISSN: 1319-0164 

24 

3 

241-244 

English 

27275108 

10.1016/j.jsps.2016.04.005 

WOS:000377389400002 

http://www.sciencedirect.com/science/article/pii/S1319016416300068
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To explore material basis of in vitro anti-proliferative activity of leukemia cell K562 of petroleum ether phase of product resulting from Galium aparine L. 60% ethanol extraction, the experiment adopts column chromatography combined with thin layer preparation, isolates and purifies petroleum ether, conducts structural identification of obtained single compound and applies MTT method for viability assay of in vitro anti-proliferative activity of leukemia cell K562. Experimental results show that G. aparine L. petroleum ether contains mainly β-sitosterol, daucosterol and dibutyl phthalate and other substances. Under experimental conditions, the three could inhibit the proliferation of leukemia cell K562 with dose-effect and time-effect relationship, of which dibutyl phthalate has strongest activity. Dibutyl phthalate with excellent activity, β-sitosterol with rich content and moderate effect should be the main contributor to its biological activity. 

L.; Purification